PMID- 22985398
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20211021
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Print)
IS  - 1096-620X (Linking)
VI  - 15
IP  - 10
DP  - 2012 Oct
TI  - Effect of Rosemarinus officinalis L. on MMP-9, MCP-1 levels, and cell migration 
      in RAW 264.7 and smooth muscle cells.
PG  - 879-86
LID - 10.1089/jmf.2012.2162 [doi]
AB  - Atherosclerosis is a chronic and progressive inflammatory disease. Novel 
      anti-inflammatory therapies may have promise as treatment strategies for 
      cardiovascular risk reduction. Rosemary (Rosemarinus officinalis L.) has been 
      used in folk medicine to treat headaches, epilepsy, poor circulation, and many 
      other ailments. It was found that rosemary could act as a stimulant and mild 
      analgesic and could reduce inflammation. However, the mechanisms underlying the 
      anti-inflammatory and antiatherosclerotic effects of rosemary need more study. 
      This study investigated effects of the rosemary components, carnosic acid (CA), 
      and carnosol (CAR), on cell migration. Monocyte chemoattractant protein-1 (MCP-1) 
      and matrix metalloproteinase-9 (MMP-9) were determined by Western blot and 
      gelatin zymography, respectively, in RAW 264.7 macrophages and vascular smooth 
      muscle cells (VSMCs). VSMC migration was assessed by a Matrigel migration assay. 
      Active compounds of rosemary extracts were also analyzed using a reversed-phase 
      high-performance liquid chromatography. MMP-9 and MCP-1 activities were markedly 
      diminished with methanol extract (RM), n-hexane fraction (RH), and CA in RAW 
      264.7 cells. RM, RH, CA, and CAR suppressed tumor necrosis factor-alpha-induced 
      VSMC migration by inhibiting MMP-9 expression. Chromatograms of RM- and 
      RH-containing CA and CAR revealed higher CA contents of RM (9.4%, 93.85 mug/mg dry 
      wt.) and, especially, RH (18.4%, 184.00 mug/mg dry wt.), which were appreciably 
      elevated compared with the similar CAR content in RM and RH (3.7%, 37.30 mug/mg 
      dry wt.; and 2.5%, 25.05 mug/mg dry wt., respectively). Rosemary, especially its 
      CA component, has potential antiatherosclerosis effects related to cell 
      migration.
FAU - Chae, In Gyeong
AU  - Chae IG
AD  - College of Pharmacy, Keimyung University, Daegu, Korea.
FAU - Yu, Mi Hee
AU  - Yu MH
FAU - Im, Nam-Kyung
AU  - Im NK
FAU - Jung, Young Tae
AU  - Jung YT
FAU - Lee, Jinho
AU  - Lee J
FAU - Chun, Kyung-Soo
AU  - Chun KS
FAU - Lee, In-Seon
AU  - Lee IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120917
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Abietanes)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 483O455CKD (carnosol)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, mouse)
RN  - LI791SXT24 (salvin)
SB  - IM
MH  - Abietanes/pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Atherosclerosis/drug therapy
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Movement/*drug effects
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Matrix Metalloproteinase 9/*genetics/metabolism
MH  - Mice
MH  - Muscle, Smooth, Vascular/cytology/drug effects
MH  - Myocytes, Smooth Muscle/*drug effects
MH  - Plant Extracts/pharmacology
MH  - Rosmarinus/*chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC3466913
EDAT- 2012/09/19 06:00
MHDA- 2013/03/15 06:00
PMCR- 2013/10/01
CRDT- 2012/09/19 06:00
PHST- 2012/09/19 06:00 [entrez]
PHST- 2012/09/19 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - 10.1089/jmf.2012.2162 [pii]
AID - 10.1089/jmf.2012.2162 [doi]
PST - ppublish
SO  - J Med Food. 2012 Oct;15(10):879-86. doi: 10.1089/jmf.2012.2162. Epub 2012 Sep 17.