PMID- 22985557
OWN - NLM
STAT- MEDLINE
DCOM- 20130725
LR  - 20161125
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 32
IP  - 9
DP  - 2012 Sep
TI  - [Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 
      release from human lung fibroblasts].
PG  - 1250-4
AB  - OBJECTIVE: To investigate the role of protein kinase C (PKC) in thrombin-induced 
      monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts 
      (HLF-1). METHODS: Cultured human lung fibroblasts HLF-1 were incubated with 
      different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l 
      protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA 
      levels in the cell lysate were measured by quantitative real-time PCR. RESULTS: 
      The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously 
      inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, 
      whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects. CONCLUSION: 
      Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 
      cells.
FAU - Yuan, Chao
AU  - Yuan C
AD  - Department of Cell Biology and Genetics, Shantou University Medical College, 
      Shantou University Medical College, Shantou 515041, China. yuanchao.10086@163.com
FAU - Xu, Mingyan
AU  - Xu M
FAU - Chen, Gengzhen
AU  - Chen G
FAU - Fu, Yucai
AU  - Fu Y
FAU - Deng, Xiaoling
AU  - Deng X
LA  - chi
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Indoles)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.4.21.5 (Thrombin)
RN  - W9A0B5E78O (Ro 31-8220)
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Fibroblasts/*metabolism
MH  - Humans
MH  - Indoles/*pharmacology
MH  - Lung/cytology/metabolism
MH  - Protein Kinase C/*antagonists & inhibitors
MH  - Thrombin/pharmacology
EDAT- 2012/09/19 06:00
MHDA- 2013/07/26 06:00
CRDT- 2012/09/19 06:00
PHST- 2012/09/19 06:00 [entrez]
PHST- 2012/09/19 06:00 [pubmed]
PHST- 2013/07/26 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1250-4.