PMID- 22988111
OWN - NLM
STAT- MEDLINE
DCOM- 20121231
LR  - 20211021
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 109
IP  - 40
DP  - 2012 Oct 2
TI  - C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through 
      interaction with the COP1 protein.
PG  - 16366-70
LID - 10.1073/pnas.1210898109 [doi]
AB  - UV-B light initiates photomorphogenic responses in plants. Arabidopsis UV 
      RESISTANCE LOCUS8 (UVR8) specifically mediates these responses by functioning as 
      a UV-B photoreceptor. UV-B exposure converts UVR8 from a dimer to a monomer, 
      stimulates the rapid accumulation of UVR8 in the nucleus, where it binds to 
      chromatin, and induces interaction of UVR8 with CONSTITUTIVELY PHOTOMORPHOGENIC1 
      (COP1), which functions with UVR8 to control photomorphogenic UV-B responses. 
      Although the crystal structure of UVR8 reveals the basis of photoreception, it 
      does not show how UVR8 initiates signaling through interaction with COP1. Here we 
      report that a region of 27 amino acids from the C terminus of UVR8 (C27) mediates 
      the interaction with COP1. The C27 region is necessary for UVR8 function in the 
      regulation of gene expression and hypocotyl growth suppression in Arabidopsis. 
      However, UVR8 lacking C27 still undergoes UV-B-induced monomerization in both 
      yeast and plant protein extracts, accumulates in the nucleus in response to UV-B, 
      and interacts with chromatin at the UVR8-regulated ELONGATED HYPOCOTYL5 (HY5) 
      gene. The UV-B-dependent interaction of UVR8 and COP1 is reproduced in yeast 
      cells and we show that C27 is both necessary and sufficient for the interaction 
      of UVR8 with the WD40 domain of COP1. Furthermore, we show that C27 interacts in 
      yeast with the REPRESSOR OF UV-B PHOTOMORPHOGENESIS proteins, RUP1 and RUP2, 
      which are negative regulators of UVR8 function. Hence the C27 region has a key 
      role in UVR8 function.
FAU - Cloix, Catherine
AU  - Cloix C
AD  - Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary 
      and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
FAU - Kaiserli, Eirini
AU  - Kaiserli E
FAU - Heilmann, Monika
AU  - Heilmann M
FAU - Baxter, Katherine J
AU  - Baxter KJ
FAU - Brown, Bobby A
AU  - Brown BA
FAU - O'Hara, Andrew
AU  - O'Hara A
FAU - Smith, Brian O
AU  - Smith BO
FAU - Christie, John M
AU  - Christie JM
FAU - Jenkins, Gareth I
AU  - Jenkins GI
LA  - eng
GR  - BB/C007727/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/F014961/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120917
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Uvr8 protein, Arabidopsis)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*physiology
MH  - Arabidopsis Proteins/*genetics/*metabolism
MH  - Chromatin Immunoprecipitation
MH  - Chromosomal Proteins, Non-Histone/*genetics/*metabolism
MH  - Gene Expression Regulation, Plant/*physiology
MH  - Green Fluorescent Proteins
MH  - Immunoprecipitation
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*physiology
MH  - Two-Hybrid System Techniques
MH  - Ubiquitin-Protein Ligases
MH  - Ultraviolet Rays
MH  - Yeasts
PMC - PMC3479605
COIS- The authors declare no conflict of interest.
EDAT- 2012/09/19 06:00
MHDA- 2013/01/01 06:00
PMCR- 2013/04/02
CRDT- 2012/09/19 06:00
PHST- 2012/09/19 06:00 [entrez]
PHST- 2012/09/19 06:00 [pubmed]
PHST- 2013/01/01 06:00 [medline]
PHST- 2013/04/02 00:00 [pmc-release]
AID - 1210898109 [pii]
AID - 201210898 [pii]
AID - 10.1073/pnas.1210898109 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16366-70. doi: 
      10.1073/pnas.1210898109. Epub 2012 Sep 17.