PMID- 22988127
OWN - NLM
STAT- MEDLINE
DCOM- 20121231
LR  - 20211021
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 109
IP  - 40
DP  - 2012 Oct 2
TI  - Features of the Arabidopsis recombination landscape resulting from the combined 
      loss of sequence variation and DNA methylation.
PG  - 16240-5
LID - 10.1073/pnas.1212955109 [doi]
AB  - The rate of meiotic crossing over (CO) varies considerably along chromosomes, 
      leading to marked distortions between physical and genetic distances. The causes 
      underlying this variation are being unraveled, and DNA sequence and chromatin 
      states have emerged as key factors. However, the extent to which the suppression 
      of COs within the repeat-rich pericentromeric regions of plant and mammalian 
      chromosomes results from their high level of DNA polymorphisms and from their 
      heterochromatic state, notably their dense DNA methylation, remains unknown. 
      Here, we test the combined effect of removing sequence polymorphisms and 
      repeat-associated DNA methylation on the meiotic recombination landscape of an 
      Arabidopsis mapping population. To do so, we use genome-wide DNA methylation data 
      from a large panel of isogenic epigenetic recombinant inbred lines (epiRILs) to 
      derive a recombination map based on 126 meiotically stable, differentially 
      methylated regions covering 81.9% of the genome. We demonstrate that the 
      suppression of COs within pericentromeric regions of chromosomes persists in this 
      experimental setting. Moreover, suppression is reinforced within 3-Mb regions 
      flanking pericentromeric boundaries, and this effect appears to be compensated by 
      increased recombination activity in chromosome arms. A direct comparison with 17 
      classical Arabidopsis crosses shows that these recombination changes place the 
      epiRILs at the boundary of the range of natural variation but are not severe 
      enough to transgress that boundary significantly. This level of robustness is 
      remarkable, considering that this population represents an extreme with key 
      recombination barriers having been forced to a minimum.
FAU - Colome-Tatche, Maria
AU  - Colome-Tatche M
AD  - Groningen Bioinformatics Centre, University of Groningen, 9747 AG Groningen, The 
      Netherlands.
FAU - Cortijo, Sandra
AU  - Cortijo S
FAU - Wardenaar, Rene
AU  - Wardenaar R
FAU - Morgado, Lionel
AU  - Morgado L
FAU - Lahouze, Benoit
AU  - Lahouze B
FAU - Sarazin, Alexis
AU  - Sarazin A
FAU - Etcheverry, Mathilde
AU  - Etcheverry M
FAU - Martin, Antoine
AU  - Martin A
FAU - Feng, Suhua
AU  - Feng S
FAU - Duvernois-Berthet, Evelyne
AU  - Duvernois-Berthet E
FAU - Labadie, Karine
AU  - Labadie K
FAU - Wincker, Patrick
AU  - Wincker P
FAU - Jacobsen, Steven E
AU  - Jacobsen SE
FAU - Jansen, Ritsert C
AU  - Jansen RC
FAU - Colot, Vincent
AU  - Colot V
FAU - Johannes, Frank
AU  - Johannes F
LA  - eng
SI  - GEO/GSE37106
SI  - GEO/GSE37284
SI  - GEO/GSE37285
GR  - R01 GM060398/GM/NIGMS NIH HHS/United States
GR  - R37 GM060398/GM/NIGMS NIH HHS/United States
GR  - GM60398/GM/NIGMS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120917
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
SB  - IM
MH  - Arabidopsis/*genetics
MH  - Crosses, Genetic
MH  - Crossing Over, Genetic/*genetics
MH  - DNA Methylation/*genetics
MH  - Epigenesis, Genetic/*genetics
MH  - Gene Expression Profiling
MH  - *Genetic Variation
PMC - PMC3479620
COIS- The authors declare no conflict of interest.
EDAT- 2012/09/19 06:00
MHDA- 2013/01/01 06:00
PMCR- 2012/09/17
CRDT- 2012/09/19 06:00
PHST- 2012/09/19 06:00 [entrez]
PHST- 2012/09/19 06:00 [pubmed]
PHST- 2013/01/01 06:00 [medline]
PHST- 2012/09/17 00:00 [pmc-release]
AID - 1212955109 [pii]
AID - 201212955 [pii]
AID - 10.1073/pnas.1212955109 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16240-5. doi: 
      10.1073/pnas.1212955109. Epub 2012 Sep 17.