PMID- 22991893
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130307
LR  - 20121016
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 84
IP  - 20
DP  - 2012 Oct 16
TI  - Capillary coated with graphene and graphene oxide sheets as stationary phase for 
      capillary electrochromatography and capillary liquid chromatography.
PG  - 8880-90
LID - 10.1021/ac3023636 [doi]
AB  - Graphene oxide (GO) nanosheets were immobilized onto the capillary wall using 
      3-aminopropyldiethoxymethyl silane as coupling agent. Graphene coated column 
      (G@column) was fabricated by hydrazine reduction of GO modified column. Scanning 
      electron microscopy (SEM) images provided visible evidence of the GO grafted on 
      the capillary wall. Energy dispersive X-ray spectrometry (EDS) indicated the high 
      coverage of the GO on the capillary wall. The G@column exhibited a pH-dependent 
      electroosmotic flow (EOF) from anode to cathode in the pH range of 3-9 while the 
      graphene oxide coated column (GO@column) showed a constant EOF. Both GO@column 
      and G@column were evaluated for open-tubular capillary electrochromatography 
      (OT-CEC). The GO@column was also evaluated for open-tubular capillary liquid 
      chromatography (OT-CLC). Good separation of the tested neutral analytes on the 
      GO@column was achieved on the basis of a typical reversed-phase behavior. On the 
      contrary, G@column showed poor separation performance because of the strong pi-pi 
      stacking and hydrophobic interactions between graphene and polyaromatic 
      hydrocarbons. The high coverage of GO improved the column phase ratio which makes 
      the GO@column promising for OT-CLC separation. Five of the major known proteins 
      including three glycoisoforms of ovalbumin in chicken egg white were identified 
      in a single run on the GO@column with phosphate buffer (5 mM, pH 7.0) and an 
      applied voltage of 20 kV. The run-to-run, day-to-day, and column-to-column 
      reproducibilities are evaluated by calculating the relative standard deviations 
      (RSDs) of the EOF in OT-CEC and retention time of naphthalene in OT-CLC, 
      respectively. These RSD values were found to be less than 3%.
FAU - Qu, Qishu
AU  - Qu Q
AD  - Jiangsu Key Laboratory of Environmental Materials and Environmental Engineering, 
      College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 
      225002, China. quqishu@qq.com
FAU - Gu, Chenhao
AU  - Gu C
FAU - Hu, Xiaoya
AU  - Hu X
LA  - eng
PT  - Journal Article
DEP - 20120928
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 2012/09/21 06:00
MHDA- 2012/09/21 06:01
CRDT- 2012/09/21 06:00
PHST- 2012/09/21 06:00 [entrez]
PHST- 2012/09/21 06:00 [pubmed]
PHST- 2012/09/21 06:01 [medline]
AID - 10.1021/ac3023636 [doi]
PST - ppublish
SO  - Anal Chem. 2012 Oct 16;84(20):8880-90. doi: 10.1021/ac3023636. Epub 2012 Sep 28.