PMID- 22991972
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20231213
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 133
IP  - 1
DP  - 2013 Jan
TI  - Early hearing protection by brain-derived neurotrophic factor.
PG  - 12-21
LID - 10.3109/00016489.2012.712217 [doi]
AB  - CONCLUSION: Brain-derived neurotrophic factor (BDNF) protects the inner ear from 
      PaExoA (exotoxin A from Pseudomonas aeruginosa)-induced sensory neural hearing 
      loss when administered 12 h after exotoxin, but not after 72 h. OBJECTIVE: BDNF 
      is a peptide in the neurotrophin family with protective effects against 
      noise-induced hair cell loss and toxic inner ear damage following exposure to 
      cisplatin. The exotoxin A (PaExoA) from P. aeruginosa, the most common 
      microorganism in chronic suppurative otitis media, induces sensorineural hearing 
      loss in rats. Previous study showed that, when given simultaneously with the 
      exotoxin, BDNF protected the inner ear from damage. The aim of this study was to 
      determine if BDNF has a protective effect when given 12-72 h after PaExoA. 
      MATERIALS AND METHODS: Five groups of Sprague-Dawley rats were used. The three 
      control groups (n = 16) were as follows. Group 1 (n = 8) received 15 microg/20 microl 
      PaExoA; group 2 (n = 5) received 20 microg/20 microl PaExoA; and group 3 (n = 3) received 
      25 microg/20 microl PaExoA injected into the round window niche. There were two treatment 
      groups (n = 12): group A (n = 6) received 15 microg/20 microl PaExoA and 4 microg/20 microl BDNF 
      12 h later; group B (n = 6) received 15 microg/20 microl PaExoA and 4 microg/20 microl BDNF 72 h 
      later. Brainstem response audiometry (ABR) was performed on day 0 (control), and 
      repeated on days 7, 14, 21, 28, and 35 to analyze the thresholds shifts. RESULTS: 
      Exposure to 15 microg/20 microl PaExoA caused persistent and significant ABR impairment 
      in controls when measured after 35 days. A single dose of BDNF given 12 h after 
      PaExoA reduced hearing loss significantly, but when BDNF was given 72 h after 
      PaExoA no protective effect was evident.
FAU - Lidian, Adnan
AU  - Lidian A
AD  - Department of Oto-Rhino-Laryngology and Head & Neck Surgery, University Hospital 
      (Akademiska sjukhuset), Uppsala, Sweden. adnan.lidian@akademiska.se
FAU - Stenkvist-Asplund, Monika
AU  - Stenkvist-Asplund M
FAU - Linder, Birgitta
AU  - Linder B
FAU - Anniko, Matti
AU  - Anniko M
FAU - Nordang, Leif
AU  - Nordang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120919
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Bacterial Toxins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Exotoxins)
RN  - 0 (Virulence Factors)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
SB  - IM
MH  - ADP Ribose Transferases
MH  - Animals
MH  - Auditory Threshold/physiology
MH  - Bacterial Toxins
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Disease Models, Animal
MH  - Drug Administration Schedule
MH  - Ear, Middle
MH  - Evoked Potentials, Auditory, Brain Stem/physiology
MH  - Exotoxins
MH  - Hearing Loss/diagnosis/*etiology/*prevention & control
MH  - Instillation, Drug
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Virulence Factors
MH  - Pseudomonas aeruginosa Exotoxin A
EDAT- 2012/09/21 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/09/21 06:00
PHST- 2012/09/21 06:00 [entrez]
PHST- 2012/09/21 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.3109/00016489.2012.712217 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2013 Jan;133(1):12-21. doi: 10.3109/00016489.2012.712217. Epub 
      2012 Sep 19.