PMID- 22993666
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 1948-5182 (Electronic)
VI  - 4
IP  - 8
DP  - 2012 Aug 27
TI  - Relationship between vitamin D and IL-23, IL-17 and macrophage chemoattractant 
      protein-1 as markers of fibrosis in hepatitis C virus Egyptians.
PG  - 242-7
AB  - AIM: To assess vitamin D in hepatitis C patients and its relationship to 
      interleukin (IL)-23, IL-17, and macrophage chemoattractant protein-1 (MCP-1). 
      METHODS: The study was conducted on 50 Egyptian hepatitis C virus (HCV) genotype 
      number IV-infected patients and 25 age- and gender-matched healthy subjects. 
      Venous blood samples were obtained. Samples were allowed to clot and sera were 
      separated by centrifugation and stored at -20  degrees C. A 25 hydroxy vitamin D assay 
      was carried out using solid phase RIA. A 1,25 dihydroxy vitamin D assay was 
      carried out using a commercial kit purchased from Incstar Corporation. IL-17 and 
      -23 and MCP-1 were assayed by an enzyme immunoassay. Quantitative and qualitative 
      polymerase chain reaction for HCV virus were done by TaqMan technology. Only HCV 
      genotype IV-infected subjects were included in the study. The mean +/- SD were 
      determined, a t-test for comparison of means of different parameters was used. 
      Correlation analysis was done using Pearson's correlation. Differences among 
      different groups were determined using the Kruskal-Wallis test. RESULTS: The mean 
      vitamin D level in HCV patients (group I) was 15 +/- 5.2 ng/mL while in control 
      (group II) was 39.7 +/- 10.8. For active vitamin D in group I as 16.6 +/- 4.8 ng/mL 
      while in group II was 41.9 +/- 7.9. IL-23 was 154 +/- 97.8 in group I and 6.7 +/- 2.17 
      in group II. IL-17 was 70.7 +/- 72.5 in cases and 1.2 +/- 0.4 in control. MCP-1 was 
      1582 +/- 794.4 in group I and 216.1 +/- 5.38 in group II. Vitamin D deficiency 
      affected 72% of HCV-infected patients and 0% of the control group. Vitamin D 
      insufficiency existed in 28% of HCV-infected patients and 12% of the control 
      group. One hundred percent of the cirrhotic patients and 40% of non cirrhotic 
      HCV-infected patients had vitamin D deficiency. IL-23, IL-17, and MCP-1 were 
      markedly increased in HCV-infected patients in comparison to controls.A 
      significant negative correlation between vitamin D and IL-17 and -23 and MCP-1 
      was detected. HCV-infected males and females showed no differences with respect 
      to viral load, vitamin D levels, IL-17, IL-23 and MCP-1. The viral load was 
      negatively correlated with vitamin D and active vitamin D (P = 0.0001 and P = 
      0.001, respectively), while positively correlated with IL-23, IL-17, and MCP-1. 
      We classified the patients according to sonar findings into four groups. Group Ia 
      with bright hepatomegaly and included 14 patients. Group Ib with perihepatic 
      fibrosis and included 11 patients. Group Ic with liver cirrhosis and included 11 
      patients. Group Id with hepatocellular carcinoma (HCC) and included 14 patients. 
      Vitamin D and active vitamin D were shown to be lower in cirrhotic patients and 
      much lower in patients with HCC, and this difference was highly significant (P = 
      0.0001). IL-17 and -23 and MCP-1 were higher in advanced liver disease) and the 
      differences were highly significant (P = 0.0001). CONCLUSION: Whether the 
      deficiency of vitamin D is related to HCV-induced chronic liver disease or 
      predisposing factor for higher viral load is a matter of debate.
FAU - El Husseiny, Noha M
AU  - El Husseiny NM
AD  - Noha M El Husseiny, Hala M Fahmy, Internal Medicine Department, Faculty of 
      Medicine, Cairo University, Cairo 11111, Egypt.
FAU - Fahmy, Hala M
AU  - Fahmy HM
FAU - Mohamed, Waleed A
AU  - Mohamed WA
FAU - Amin, Hisham H
AU  - Amin HH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Hepatol
JT  - World journal of hepatology
JID - 101532469
PMC - PMC3443706
OTO - NOTNLM
OT  - Interleukin-17
OT  - Interleukin-23
OT  - Liver cirrhosis
OT  - Macrophage chemoattractant protin-1
OT  - Vitamin D
EDAT- 2012/09/21 06:00
MHDA- 2012/09/21 06:01
PMCR- 2012/08/27
CRDT- 2012/09/21 06:00
PHST- 2012/01/01 00:00 [received]
PHST- 2012/08/06 00:00 [revised]
PHST- 2012/08/23 00:00 [accepted]
PHST- 2012/09/21 06:00 [entrez]
PHST- 2012/09/21 06:00 [pubmed]
PHST- 2012/09/21 06:01 [medline]
PHST- 2012/08/27 00:00 [pmc-release]
AID - 10.4254/wjh.v4.i8.242 [doi]
PST - ppublish
SO  - World J Hepatol. 2012 Aug 27;4(8):242-7. doi: 10.4254/wjh.v4.i8.242.