PMID- 22995218
OWN - NLM
STAT- MEDLINE
DCOM- 20131224
LR  - 20200930
IS  - 1873-2690 (Electronic)
IS  - 0981-9428 (Linking)
VI  - 60
DP  - 2012 Nov
TI  - Proteome analysis of the large and the small rubber particles of Hevea 
      brasiliensis using 2D-DIGE.
PG  - 207-13
LID - S0981-9428(12)00238-0 [pii]
LID - 10.1016/j.plaphy.2012.08.010 [doi]
AB  - The rubber particle is a specialized organelle in which natural rubber is 
      synthesised and stored in the laticifers of Hevea brasiliensis (para rubber 
      tree). It has been demonstrated that the small rubber particles (SRPs) has higher 
      rubber biosynthesis ratio than the large rubber particles (LRPs), but the 
      underlying molecular mechanism still remains unknown. In this study, LRPs and 
      SRPs were firstly separated from the fresh latex using differential 
      centrifugation, and two-dimensional difference in-gel electrophoresis (2D-DIGE) 
      combined with MALDI-TOF/TOF was then applied to investigate the proteomic 
      alterations associated with the changed rubber biosynthesis capacity between LRPs 
      and SRPs. A total of 53 spots corresponding to 22 gene products, were 
      significantly altered with the |ratio|>/=2.0 and T value </=0.05, among which 15 
      proteins were up-regulated and 7 were down-regulated in the SRPs compared with 
      the LRPs. The 15 up-regulated proteins in the SRPs included small rubber particle 
      protein (SRPP), 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS), phospholipase D 
      alpha (PLD alpha), ethylene response factor 2, eukaryotic translation initiation 
      factor 5A isoform IV (eIF 5A-4), 70-kDa heat shock cognate protein (HSC 70), 
      several unknown proteins, etc., whereas the 7 up-regulated proteins in the LRPs 
      were rubber elongation factor (REF, 19.6kDa), ASR-like protein 1, REF-like 
      stress-related protein 1, a putative phosphoglyceride transfer family protein, 
      beta-1,3-glucanase, a putative retroelement, and a hypothetical protein. Since 
      several proteins related to rubber biosynthesis were differentially expressed 
      between LRPs and SRPs, the comparative proteome data may provide useful insights 
      into understanding the mechanism involved in rubber biosynthesis and latex 
      coagulation in H. brasiliensis.
CI  - Copyright (c) 2012 Elsevier Masson SAS. All rights reserved.
FAU - Xiang, Qiulan
AU  - Xiang Q
AD  - Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of 
      Agriculture, PR China. alan-42@163.com
FAU - Xia, Kecan
AU  - Xia K
FAU - Dai, Longjun
AU  - Dai L
FAU - Kang, Guijuan
AU  - Kang G
FAU - Li, Yu
AU  - Li Y
FAU - Nie, Zhiyi
AU  - Nie Z
FAU - Duan, Cuifang
AU  - Duan C
FAU - Zeng, Rizhong
AU  - Zeng R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120905
PL  - France
TA  - Plant Physiol Biochem
JT  - Plant physiology and biochemistry : PPB
JID - 9882449
RN  - 0 (Latex)
RN  - 0 (Plant Proteins)
RN  - 0 (Proteome)
RN  - 9006-04-6 (Rubber)
SB  - IM
MH  - Down-Regulation
MH  - Hevea/*chemistry/metabolism/ultrastructure
MH  - Latex/*isolation & purification/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Organelles/metabolism/ultrastructure
MH  - Plant Proteins/*isolation & purification/metabolism
MH  - Proteome
MH  - Proteomics
MH  - Rubber/*isolation & purification/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Two-Dimensional Difference Gel Electrophoresis/*methods
MH  - Up-Regulation
EDAT- 2012/09/22 06:00
MHDA- 2013/12/25 06:00
CRDT- 2012/09/22 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2012/08/23 00:00 [accepted]
PHST- 2012/09/22 06:00 [entrez]
PHST- 2012/09/22 06:00 [pubmed]
PHST- 2013/12/25 06:00 [medline]
AID - S0981-9428(12)00238-0 [pii]
AID - 10.1016/j.plaphy.2012.08.010 [doi]
PST - ppublish
SO  - Plant Physiol Biochem. 2012 Nov;60:207-13. doi: 10.1016/j.plaphy.2012.08.010. 
      Epub 2012 Sep 5.