PMID- 22995314
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20121123
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 428
IP  - 3
DP  - 2012 Nov 23
TI  - Role of RNA-binding protein tristetraprolin in tumor necrosis factor-alpha mediated 
      gene expression.
PG  - 327-32
LID - S0006-291X(12)01773-1 [pii]
LID - 10.1016/j.bbrc.2012.09.033 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays an important role in the pathogenesis of 
      inflammatory diseases. Excessive TNF-alpha expression induces tristetraprolin (TTP), 
      an RNA-binding protein that regulates mRNA degradation, which in turn 
      downregulates TNF and its downstream genes, thus resulting in anti-inflammatory 
      effects. In order to better understand the TNF-alpha mediated molecular pathways in 
      inflammatory diseases, embryonic fibroblast (MEF) cell lines derived from 
      TTP-deficient (KO) or wild type (WT) mice were treated with TNF-alpha and gene 
      expression differences between two cell lines were compared by a microarray essay 
      of 9224 genes. We found that TTP-KO cells had higher expression levels of 
      pro-inflammatory genes than TTP-WT cells, and inflammatory genes were 
      differentially regulated by TNF-alpha between TTP-KO and TTP-WT cells. Through a 
      study of 2-dimentional gene set matrix analysis, we also found the genes 
      upregulated by TNF-alpha in TTP KO cells were correlated with the pathologic 
      phenotypes in inflammation, joint, or bone diseases. Our study provided a 
      detailed genetic roadmap for further understanding the regulatory effect of TTP 
      in inflammatory pathways related to human diseases.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Chen, Xia
AU  - Chen X
AD  - Urology Department, Minhang District Central Hospital, Shanghai, China.
FAU - Wei, Ziran
AU  - Wei Z
FAU - Wang, Weimin
AU  - Wang W
FAU - Yan, Ronlin
AU  - Yan R
FAU - Xu, Xudong
AU  - Xu X
FAU - Cai, Qingping
AU  - Cai Q
LA  - eng
PT  - Journal Article
DEP - 20120917
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Tristetraprolin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Zfp36 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Inflammation/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - RNA-Binding Proteins/genetics/*physiology
MH  - Tristetraprolin/genetics/*physiology
MH  - Tumor Necrosis Factor-alpha/pharmacology/*physiology
EDAT- 2012/09/22 06:00
MHDA- 2013/03/26 06:00
CRDT- 2012/09/22 06:00
PHST- 2012/08/20 00:00 [received]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/09/22 06:00 [entrez]
PHST- 2012/09/22 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
AID - S0006-291X(12)01773-1 [pii]
AID - 10.1016/j.bbrc.2012.09.033 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Nov 23;428(3):327-32. doi: 
      10.1016/j.bbrc.2012.09.033. Epub 2012 Sep 17.