PMID- 22996307
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20231213
IS  - 1520-636X (Electronic)
IS  - 0899-0042 (Linking)
VI  - 25
IP  - 1
DP  - 2013 Jan
TI  - Selective regulation of nicotine and polyamines biosynthesis in tobacco cells by 
      enantiomers of ornithine.
PG  - 22-7
LID - 10.1002/chir.22107 [doi]
AB  - L- and D-amino acids have diverse functions and effects on the metabolism, 
      growth, and development of plants. Ornithine (Orn) plays a main role in the 
      biosynthesis of many amino acids, nicotinic alkaloids, and polyamines in tobacco. 
      This investigation describes the impact of Orn enantiomers on the production and 
      distribution of free, conjugated, and bound polyamines, as well as nicotine in 
      tobacco cells. It was recognized that the biosynthesis of metabolites was 
      differently upregulated by each enantiomer. Putrescine was abundantly produced by 
      exogenous L-ornithine (L-Orn), and both spermidine and spermine were 
      significantly accumulated in D-ornithine (D-Orn)-supplied tobacco cells. 
      Furthermore, nicotine production was highly upregulated by L-Orn, while the 
      addition of D-Orn had no effect on the nicotine content of tobacco cells. It was 
      observed that transcript expression of S-adenosylmethionine decarboxylase, as the 
      key enzyme of spermidine/spermine biosynthesis, is coincident with their 
      metabolic levels and is highly upregulated by D-Orn, as opposed to L-Orn. These 
      results indicate that both enantiomers of Orn can trigger selected biosynthetic 
      pathways in the cells, at the transcript level. Regarding these observations, it 
      is proposed that L- and D-Orn function differently in the same biological 
      pathways in which the latter, D-Orn specifically regulates important polyamines 
      in the plant cells.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Gholami, Morteza
AU  - Gholami M
AD  - Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., 
      Tehran, Iran.
FAU - Fakhari, Ali Reza
AU  - Fakhari AR
FAU - Ghanati, Faezeh
AU  - Ghanati F
LA  - eng
PT  - Journal Article
DEP - 20120919
PL  - United States
TA  - Chirality
JT  - Chirality
JID - 8914261
RN  - 0 (Biogenic Polyamines)
RN  - 6M3C89ZY6R (Nicotine)
RN  - E524N2IXA3 (Ornithine)
SB  - IM
MH  - Biogenic Polyamines/*biosynthesis
MH  - Cells, Cultured
MH  - Nicotine/*biosynthesis
MH  - Ornithine/*pharmacology
MH  - Stereoisomerism
MH  - Nicotiana/cytology/*metabolism
EDAT- 2012/09/22 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/09/22 06:00
PHST- 2012/05/06 00:00 [received]
PHST- 2012/07/12 00:00 [accepted]
PHST- 2012/09/22 06:00 [entrez]
PHST- 2012/09/22 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
AID - 10.1002/chir.22107 [doi]
PST - ppublish
SO  - Chirality. 2013 Jan;25(1):22-7. doi: 10.1002/chir.22107. Epub 2012 Sep 19.