PMID- 22996438
OWN - NLM
STAT- MEDLINE
DCOM- 20131114
LR  - 20191210
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 38
IP  - 6
DP  - 2012 Nov-Dec
TI  - In vitro evaluation of the fermentation properties and potential prebiotic 
      activity of caprine cheese whey oligosaccharides in batch culture systems.
PG  - 440-9
LID - 10.1002/biof.1043 [doi]
AB  - The prebiotic effect of oligosaccharides recovered and purified from caprine 
      whey, was evaluated by in vitro fermentation under anaerobic conditions using 
      batch cultures at 37 degrees C with human faeces. Effects on key gut bacterial groups 
      were monitored over 24 h by fluorescence in situ hybridization (FISH), which was 
      used to determine a quantitative prebiotic index score. Production of short-chain 
      fatty acids (SCFAs) as fermentation end products was analyzed by high-performance 
      liquid chromatography (HPLC). Growth of Bifidobacterium spp was significantly 
      higher (P >/= 0.05) with the purified oligosaccharides compared to the negative 
      control. Lactic and propionic acids were the main SCFAs produced. Antimicrobial 
      activity of the oligosaccharides was also tested, revealing no inhibition though 
      a decrease in Staphylococcus aureus and Escherichia coli growth. These findings 
      indicate that naturally extracted oligosaccharides from caprine whey could be 
      used as new and valuable source of prebiotics.
CI  - Copyright (c) 2012 International Union of Biochemistry and Molecular Biology, Inc.
FAU - Oliveira, Diana L
AU  - Oliveira DL
AD  - Department of Food and Nutritional Sciences, University of Reading, Reading RG6 
      6AP, UK.
FAU - Costabile, Adele
AU  - Costabile A
FAU - Wilbey, R Andrew
AU  - Wilbey RA
FAU - Grandison, Alistair S
AU  - Grandison AS
FAU - Duarte, Luis C
AU  - Duarte LC
FAU - Roseiro, Luisa B
AU  - Roseiro LB
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120920
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Fatty Acids, Volatile)
RN  - 0 (Oligosaccharides)
RN  - 0 (Prebiotics)
SB  - IM
MH  - Animals
MH  - *Batch Cell Culture Techniques
MH  - Bifidobacterium/growth & development
MH  - Cheese/*microbiology
MH  - Dairy Products/microbiology
MH  - Fatty Acids, Volatile/chemistry
MH  - *Fermentation
MH  - Gastrointestinal Tract/microbiology
MH  - Goats
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Oligosaccharides/*chemistry
MH  - *Prebiotics
EDAT- 2012/09/22 06:00
MHDA- 2013/11/15 06:00
CRDT- 2012/09/22 06:00
PHST- 2012/04/22 00:00 [received]
PHST- 2012/08/10 00:00 [accepted]
PHST- 2012/09/22 06:00 [entrez]
PHST- 2012/09/22 06:00 [pubmed]
PHST- 2013/11/15 06:00 [medline]
AID - 10.1002/biof.1043 [doi]
PST - ppublish
SO  - Biofactors. 2012 Nov-Dec;38(6):440-9. doi: 10.1002/biof.1043. Epub 2012 Sep 20.