PMID- 22996641 OWN - NLM STAT- MEDLINE DCOM- 20140624 LR - 20140506 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 159 IP - 5 DP - 2014 May TI - Reston virus in domestic pigs in China. PG - 1129-32 LID - 10.1007/s00705-012-1477-6 [doi] AB - Historically, Reston virus (RESTV) has been found to be associated with outbreaks of disease only in nonhuman primates. Its spread to domestic pigs was reported for the first time in 2008. In this study, we report the discovery, molecular detection, and phylogenetic analysis of Reston virus (RESTV) in domestic pigs in China. A total of 137 spleen specimens from pigs that died after showing typical clinical signs of porcine reproductive and respiratory syndrome (PRRS), and for which infection with porcine reproductive and respiratory syndrome virus (PRRSV) was confirmed by RT-PCR, were collected from three farms in Shanghai from February to September 2011. Of these samples, 2.92 % (4/137) were found to be positive for RESTV. All of the positive piglets were under the age of 8 weeks and were co-infected with PRRSV. Sequences were found that shared 96.1 %-98.9 % sequence similarity with those of two RESTV variants that had been discovered previously in domestic pigs and cynomolgus macaques from the Philippines. We therefore conclude that RESTV was present in domestic pigs in Shanghai, China. FAU - Pan, Yangyang AU - Pan Y AD - The College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, People's Republic of China, panyangyang_2007@126.com. FAU - Zhang, Wen AU - Zhang W FAU - Cui, Li AU - Cui L FAU - Hua, Xiuguo AU - Hua X FAU - Wang, Meng AU - Wang M FAU - Zeng, Qiaoying AU - Zeng Q LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120921 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 SB - IM MH - Animals MH - China/epidemiology MH - Ebolavirus/genetics/*isolation & purification MH - Hemorrhagic Fever, Ebola/epidemiology/*veterinary/virology MH - Phylogeny MH - Swine MH - Swine Diseases/epidemiology/*virology EDAT- 2012/09/22 06:00 MHDA- 2014/06/25 06:00 CRDT- 2012/09/22 06:00 PHST- 2012/08/01 00:00 [received] PHST- 2012/08/29 00:00 [accepted] PHST- 2012/09/22 06:00 [entrez] PHST- 2012/09/22 06:00 [pubmed] PHST- 2014/06/25 06:00 [medline] AID - 10.1007/s00705-012-1477-6 [doi] PST - ppublish SO - Arch Virol. 2014 May;159(5):1129-32. doi: 10.1007/s00705-012-1477-6. Epub 2012 Sep 21.