PMID- 22997976
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20141006
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 58
IP  - 7-8
DP  - 2012
TI  - Levels of metalloproteinase (MMP-3, MMP-9), NF-kappaB ligand (RANKL), and nitric 
      oxide (NO) in peripheral blood of osteoarthritis (OA) patients.
PG  - 755-62
AB  - BACKGROUND: The aim of this study was to judge whether there is a correlation 
      between some biochemical features of knee osteoarthritic blood and clinical 
      characteristics and to evaluate the potential relationship between osteoarthitis 
      (OA) severity and putative biomarkers for the disease. METHODS: 105 patients 
      suffering from knee OA were analyzed clinically (Lequesne's index) and 
      radiographically (Kellgren and Lawrence, K&L). Plasma and peripheral blood 
      mononuclear cells (PBMC) were harvested separately. Specimens were analyzed for 
      concentrations of nitric oxide (NO), matrix metalloproteinase-3 (MMP-3), and 
      matrix metalloproteinase-9 (MMP-9). Transcript levels of the receptor activator 
      of NF-kB ligand (RANKL) mRNA, MMP-3mRNA, and MMP-9mRNA were measured using 
      real-time quantitative RT-PCR. RESULTS: Data certified significantly increasing 
      concentrations of plasma MMP-3, MMP-9, and NO as well as transcript levels of 
      RANKL mRNA and MMP-9 mRNA in early OA (at grade I). There was a positive 
      correlation of MMP-3 and MMP-9 content in plasma and MMP-9 mRNA expression levels 
      in PBMC with the severity of clinical symptoms (total Lequesne's scores) in early 
      OA. NO content in plasma correlated with total Lequesne's scores, pain scores in 
      response to pressure, and swelling scores of early OA patients (atgGrade I). 
      Analogously, there were positive correlations of RANKL mRNA expression with total 
      Lequesne's scores, pain scores in response to pressure, and swelling scores in OA 
      patients at Grade I. CONCLUSIONS: [corrected] Some biochemical factors, including 
      content of NO, MMP-3, MMP-9, and transcript levels of some genes, including MMP-9 
      mRNA and RANKL mRNA, may be specific and sensitive enough to diagnose OA diseases 
      at an early stage in the pathological process of OA when radiological features do 
      not reflect degradation of articular cartilage. Therefore, proper regulation of 
      these factors may be a promising and realistic new target for the treatment of 
      degenerative osteoarticular diseases.
FAU - Li, Heng
AU  - Li H
AD  - Department of Orthopaedics, Affiliated First People's Hospital of Huzhou Teachers 
      College, Zhejiang Province, China, 313000. lihengunion@yahoo.com.cn
FAU - Li, Liqin
AU  - Li L
FAU - Min, Jikang
AU  - Min J
FAU - Yang, Honghang
AU  - Yang H
FAU - Xu, Xuchun
AU  - Xu X
FAU - Yuan, Yongjian
AU  - Yuan Y
FAU - Wang, Dan
AU  - Wang D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (TNFSF11 protein, human)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Aged
MH  - Female
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/*blood/genetics
MH  - Matrix Metalloproteinase 9/*blood/genetics
MH  - Middle Aged
MH  - Nitric Oxide/*blood
MH  - Osteoarthritis/*blood
MH  - RANK Ligand/*blood/genetics
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2012/09/25 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
PST - ppublish
SO  - Clin Lab. 2012;58(7-8):755-62.