PMID- 23000062
OWN - NLM
STAT- MEDLINE
DCOM- 20130722
LR  - 20181202
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 74
IP  - 3-4
DP  - 2012 Dec
TI  - Pleiotrophin promotes microglia proliferation and secretion of neurotrophic 
      factors by activating extracellular signal-regulated kinase 1/2 pathway.
PG  - 269-76
LID - S0168-0102(12)00173-3 [pii]
LID - 10.1016/j.neures.2012.09.001 [doi]
AB  - Pleiotrophin (PTN) is an effective neuroprotective factor and its expression is 
      strikingly increased in microglia after ischemia/reperfusion injury. However, 
      whether PTN could provide neurotrophic support to neurons by regulating microglia 
      function is not clear. In this study, we demonstrated that the expression of PTN 
      was induced in microglia after oxygen-glucose deprivation/reperfusion. PTN 
      promoted the proliferation of microglia by enhancing the G1 to S phase 
      transition. PTN also stimulated the secretion of brain-derived neurotrophic 
      factor (BDNF), ciliary neurotrophic factor (CNTF) and nerve growth factor (NGF) 
      in microglia, but did not upregulate the expression of proinflammatory factors 
      such as TNF-alpha, IL-1beta and iNOS. Mechanistically, we found that PTN increased the 
      phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 in microglia 
      in both concentration-dependent and time-dependent manners. In addition, ERK1/2 
      inhibitor U0126 abolished the proliferation and G1 to S phase transition of 
      microglia stimulated by PTN, and inhibited the production of BDNF, CNTF and NGF 
      induced by PTN. In conclusion, our results demonstrated that PTN-ERK1/2 pathway 
      plays important role in regulating microglia growth and secretion of neurotrophic 
      factors. These findings provide new insight into the neuroprotective role of PTN 
      and suggest that PTN is a new target for therapeutic intervention of stroke.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All 
      rights reserved.
FAU - Miao, Jiayin
AU  - Miao J
AD  - Department of Neurology, First Affiliated Hospital of Sun Yat-Sen University, The 
      2nd Zhongshan Road, GuangZhou 510080, China.
FAU - Ding, Minghui
AU  - Ding M
FAU - Zhang, Aiwu
AU  - Zhang A
FAU - Xiao, Zijian
AU  - Xiao Z
FAU - Qi, Weiwei
AU  - Qi W
FAU - Luo, Ning
AU  - Luo N
FAU - Di, Wei
AU  - Di W
FAU - Tao, Yuqian
AU  - Tao Y
FAU - Fang, Yannan
AU  - Fang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120918
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Nerve Growth Factors)
RN  - 134034-50-7 (pleiotrophin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carrier Proteins/*metabolism
MH  - Cell Proliferation
MH  - Cytokines/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunohistochemistry
MH  - MAP Kinase Signaling System/*physiology
MH  - Microglia/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2012/09/25 06:00
MHDA- 2013/07/23 06:00
CRDT- 2012/09/25 06:00
PHST- 2012/07/12 00:00 [received]
PHST- 2012/08/23 00:00 [revised]
PHST- 2012/09/03 00:00 [accepted]
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/07/23 06:00 [medline]
AID - S0168-0102(12)00173-3 [pii]
AID - 10.1016/j.neures.2012.09.001 [doi]
PST - ppublish
SO  - Neurosci Res. 2012 Dec;74(3-4):269-76. doi: 10.1016/j.neures.2012.09.001. Epub 
      2012 Sep 18.