PMID- 23000510
OWN - NLM
STAT- MEDLINE
DCOM- 20130214
LR  - 20211203
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 93
IP  - 3
DP  - 2012 Dec
TI  - The role of TRB3 in mast cells sensitized with monomeric IgE.
PG  - 408-15
LID - S0014-4800(12)00130-X [pii]
LID - 10.1016/j.yexmp.2012.09.008 [doi]
AB  - Mast cells play a key role in immunoglobulin E (IgE)-associated allergic 
      disorders; however, the cellular effects of sensitization remain poorly 
      understood. Using gene microarrays and the multiplexing ELISA method, we examined 
      the effects of sensitization on RBL-CCR1 cell transcription and 
      chemokine/cytokine secretion. Sensitization most prominently increased 
      transcription of Trb3, the gene for tribbles homolog 3 (TRB3), and also increased 
      the release of most of the cytokines and chemokines tested. Knockdown of TRB3 via 
      RNAi significantly induced the production of tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-4 (IL-4), interleukin-6 (IL-6), and the chemokine mast cell 
      protease-1 (MCP-1). TRB3 deficiency also induced IkappaBalpha phosphorylation. TRB3 may 
      therefore serve as a negative regulator of pro-inflammatory cytokines and 
      chemokines, controlling the extent of the inflammatory response.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Kuo, Chuan-Hui
AU  - Kuo CH
AD  - Division of Allergy and Immunology, Department of Pediatrics, Cincinnati 
      Children's Hospital Medical Center, OH 45221-0097, USA.
FAU - Morohoshi, Kei
AU  - Morohoshi K
FAU - Aye, Cho Cho
AU  - Aye CC
FAU - Garoon, Robert B
AU  - Garoon RB
FAU - Collins, Andrea
AU  - Collins A
FAU - Ono, Santa Jeremy
AU  - Ono SJ
LA  - eng
GR  - R01 EY019630/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20120921
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Chemokines)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Trib3 protein, rat)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Chemokines/biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Mast Cells/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Protein Serine-Threonine Kinases/*antagonists & inhibitors/physiology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Rats
EDAT- 2012/09/25 06:00
MHDA- 2013/02/15 06:00
CRDT- 2012/09/25 06:00
PHST- 2012/09/13 00:00 [received]
PHST- 2012/09/14 00:00 [accepted]
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/02/15 06:00 [medline]
AID - S0014-4800(12)00130-X [pii]
AID - 10.1016/j.yexmp.2012.09.008 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2012 Dec;93(3):408-15. doi: 10.1016/j.yexmp.2012.09.008. Epub 
      2012 Sep 21.