PMID- 23001074
OWN - NLM
STAT- MEDLINE
DCOM- 20130305
LR  - 20220129
IS  - 1528-1140 (Electronic)
IS  - 0003-4932 (Linking)
VI  - 257
IP  - 2
DP  - 2013 Feb
TI  - Murine gut microbiota and transcriptome are diet dependent.
PG  - 287-94
LID - 10.1097/SLA.0b013e318262a6a6 [doi]
AB  - OBJECTIVE: Here, we determine how formula feeding impacts the gut microbiota and 
      host transcriptome. BACKGROUND: Formula-fed (FF) infants are at risk for diseases 
      that involve complex interactions between microbes and host immune elements such 
      as necrotizing enterocolitis. The aims of this study were to simultaneously 
      examine the microbiota and host transcriptional profiles of FF and maternal-fed 
      (MF) mice to evaluate how diet impacts gut colonization and host genes. METHODS: 
      After 72 hours of FF or MF, colonic tissue was collected. 16S ribosomal RNA was 
      sequenced with Roche GS-FLX (Genome Sequencer-FLX) pyrosequencing. Operational 
      taxonomical unit clustering, diversity analysis, and principal coordinate 
      analysis (PCA) were performed. Complementary DNA libraries were sequenced by 
      Solexa. Reads were annotated by BLAST (Basic Local Alignment Search Tool) search 
      against mouse RNA database [National Center for Biotechnology Information (NCBI) 
      build-37] and functionally classified using the KOG (Eukaryotic Orthologous 
      Groups) database (NCBI). RESULTS: Firmicutes (P < 0.001) was the dominant phylum 
      in MF pups, whereas Proteobacteria (P < 0.001) and Bacteroidetes (P < 0.05) were 
      dominant in FF mice. On the genus level, FF mice had increased Serratia (P < 
      0.001) and Lactococcus (P < 0.05) whereas MF mice had increased Lactobacillus (P 
      < 0.001). PCA confirmed clustering by diet. Solexa sequencing demonstrated 
      different (P < 0.05) messenger RNA transcript levels in 148 genes. Heme oxygenase 
      1 (P < 0.01), an oxidative stress marker, was increased 25-fold in FF mice. In 
      addition, decreased vinculin (P < 0.05), a cytoskeletal protein associated with 
      adherens junctions in FF pups suggested impaired gut structural integrity. Diet 
      also impacted immune regulation, cell cycle control/gene expression, cell 
      motility, and vascular function genes. CONCLUSIONS: FF shifted gut microbiota and 
      structural integrity, oxidative stress, and immune function genes, presumably 
      increasing vulnerability to disease in FF mice. Interrogation of microbial and 
      host gene expression in FF neonates may offer new insight on how diet affects 
      disease pathogenesis.
FAU - Carlisle, Erica M
AU  - Carlisle EM
AD  - Department of Surgery, The University of Chicago Medical Center, Chicago, IL 
      60637, USA.
FAU - Poroyko, Valeriy
AU  - Poroyko V
FAU - Caplan, Michael S
AU  - Caplan MS
FAU - Alverdy, John
AU  - Alverdy J
FAU - Morowitz, Michael J
AU  - Morowitz MJ
FAU - Liu, Donald
AU  - Liu D
LA  - eng
GR  - R21 AI099713/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Surg
JT  - Annals of surgery
JID - 0372354
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Animals, Newborn
MH  - Colon/*immunology/*microbiology
MH  - DNA, Complementary/genetics
MH  - Gene Library
MH  - Intestinal Mucosa/immunology
MH  - Metagenome/immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - *Milk Substitutes
MH  - *Milk, Human
MH  - Oxidative Stress/immunology
MH  - Principal Component Analysis
MH  - Receptors, Chemokine/metabolism
MH  - Transcriptome
EDAT- 2012/09/25 06:00
MHDA- 2013/03/06 06:00
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/03/06 06:00 [medline]
AID - 10.1097/SLA.0b013e318262a6a6 [doi]
PST - ppublish
SO  - Ann Surg. 2013 Feb;257(2):287-94. doi: 10.1097/SLA.0b013e318262a6a6.