PMID- 23001445
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20211021
IS  - 1745-7262 (Electronic)
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 14
IP  - 6
DP  - 2012 Nov
TI  - Downregulation of cold-inducible RNA-binding protein activates mitogen-activated 
      protein kinases and impairs spermatogenic function in mouse testes.
PG  - 884-9
LID - 10.1038/aja.2012.71 [doi]
AB  - Cold-inducible RNA-binding protein (CIRP) is an RNA-binding protein that is 
      expressed in normal testes and downregulated after heat stress caused by 
      cryptorchidism, varicocele or environmental temperatures. The purpose of this 
      study was to investigate the functions of CIRP in the testes. We employed RNAi 
      technique to knock down the expression of CIRP in the testes, and performed 
      haematoxylin and eosin staining to evaluate morphological changes following 
      knockdown. Germ cell apoptosis was examined by terminal deoxynucleotidal 
      transferase-mediated dUTP nick end labelling (TUNEL) assay, and mitogen-activated 
      protein kinase (MAPK) signalling pathways were investigated by Western blotting 
      to determine the possible mechanism of apoptosis. We found that using siRNA is a 
      feasible and reliable method for knocking down gene expression in the testes. 
      Compared to controls, the mean seminiferous tubule diameter (MSTD) and the 
      thickness of the germ cell layers decreased following siRNA treatment, whereas 
      the percentage of apoptotic seminiferous tubules increased. The p44/p42, p38 and 
      SAPK/JNK MAPK pathways were activated after downregulation of CIRP. In 
      conclusion, we discovered that downregulation of CIRP resulted in increased germ 
      cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK 
      pathways.
FAU - Xia, Zhi-Ping
AU  - Xia ZP
AD  - Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071, 
      China.
FAU - Zheng, Xin-Min
AU  - Zheng XM
FAU - Zheng, Hang
AU  - Zheng H
FAU - Liu, Xiao-Jun
AU  - Liu XJ
FAU - Liu, Gui-Yong
AU  - Liu GY
FAU - Wang, Xing-Huan
AU  - Wang XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120924
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Cirbp protein, mouse)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cold Temperature
MH  - Down-Regulation
MH  - Enzyme Activation/drug effects
MH  - Gene Knockdown Techniques/methods
MH  - MAP Kinase Signaling System/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Spermatogenesis
MH  - Testis/*metabolism
PMC - PMC3720101
EDAT- 2012/09/25 06:00
MHDA- 2013/04/23 06:00
PMCR- 2012/11/01
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - aja201271 [pii]
AID - 10.1038/aja.2012.71 [doi]
PST - ppublish
SO  - Asian J Androl. 2012 Nov;14(6):884-9. doi: 10.1038/aja.2012.71. Epub 2012 Sep 24.