PMID- 23001982
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20211021
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 26
IP  - 5
DP  - 2012 Sep
TI  - Alterations of dendritic cell subsets and TH1/TH2 cytokines in the peripheral 
      circulation of patients with superficial transitional cell carcinoma of the 
      bladder.
PG  - 365-71
LID - 10.1002/jcla.21532 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) and cytokines play an important role in the 
      tumor growth and recurrence. METHODS: Sixty-six patients with superficial 
      transitional cell carcinoma of the bladder (STCCB) and 38 healthy controls were 
      studied to investigate the percentages of DC subsets, monocyte-derived DC (MoDC) 
      function, and alterations of Th1 and Th2 cytokines. MoDCs were generated and 
      three-color flow cytometry was used for determining the phenotype of MoDCs and DC 
      subsets. The ability to stimulate autologous T cells was tested in mixed 
      leukocyte reaction (MLR). The levels of various cytokines were measured using 
      commercially available sandwich enzyme linked immunosorbent assay (ELISA) kit. 
      RESULTS: The myeloid DC (mDC) counts, MoDC surface molecular expression, and 
      stimulatory capacity to T cells were impaired in STCCB patients than in controls. 
      The percentage of mDC and the expression of CD80, CD83, and CD86 were lower in 
      patients showing recurrence. The serum levels of IL-2 and IFN-gamma were found to be 
      significantly lower while IL-4, IL-6, and IL-10 were significantly higher in 
      STCCB patients than in controls. IL-6 was found to be significantly higher in 
      recurrent patients. CONCLUSION: The impairment of mDC counts and MoDC function 
      with imbalance of Th1/Th2 cytokines was closely associated with proliferation and 
      recurrence of STCCB.
CI  - (c) 2012 Wiley Periodicals, Inc.
FAU - Lang, Feng
AU  - Lang F
AD  - Department of Urology, Beijing Friendship Hospital, Capital Medical University, 
      Beijing, China.
FAU - Linlin, Ma
AU  - Linlin M
FAU - Ye, Tian
AU  - Ye T
FAU - Yuhai, Zhang
AU  - Yuhai Z
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Analysis of Variance
MH  - Carcinoma, Transitional Cell/*blood/immunology
MH  - Case-Control Studies
MH  - Cytokines/*blood/immunology
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/blood/immunology
MH  - Phenotype
MH  - Th1 Cells/immunology/pathology
MH  - Th2 Cells/immunology/pathology
MH  - Urinary Bladder Neoplasms/*blood/immunology
PMC - PMC6807352
EDAT- 2012/09/25 06:00
MHDA- 2013/04/23 06:00
PMCR- 2012/09/21
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
PHST- 2012/09/21 00:00 [pmc-release]
AID - JCLA21532 [pii]
AID - 10.1002/jcla.21532 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2012 Sep;26(5):365-71. doi: 10.1002/jcla.21532.