PMID- 23002394
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231104
IS  - 1744-6848 (Electronic)
IS  - 1744-683X (Print)
IS  - 1744-683X (Linking)
VI  - 8
IP  - 27
DP  - 2012 Jul 21
TI  - A Novel Technique for Micro-patterning Proteins and Cells on Polyacrylamide Gels.
PG  - 7197-7206
AB  - Spatial patterning of proteins (extracellular matrix, ECM) for living cells on 
      polyacrylamide (PA) hydrogels has been technically challenging due to the 
      compliant nature of the hydrogels and their aqueous environment. Traditional 
      micro-fabrication process is not applicable. Here we report a simple, novel and 
      general method to pattern a variety of commonly used cell adhesion molecules, 
      i.e. Fibronectin (FN), Laminin (LN) and Collagen I (CN), etc. on PA gels. The 
      pattern is first printed on a hydrophilic glass using polydimethylsiloxane (PDMS) 
      stamp and micro-contact printing (muCP). Pre-polymerization solution is applied on 
      the patterned glass and is then sandwiched by a functionalized glass slide, which 
      covalently binds to the gel. The hydrophilic glass slide is then peeled off from 
      the gel when the protein patterns detach from the glass, but remain intact with 
      the gel. The pattern is thus transferred to the gel. The mechanism of pattern 
      transfer is studied in light of interfacial mechanics. It is found that 
      hydrophilic glass offers strong enough adhesion with ECM proteins such that a 
      pattern can be printed, but weak enough adhesion such that they can be completely 
      peeled off by the polymerized gel. This balance is essential for successful 
      pattern transfer. As a demonstration, lines of FN, LN and CN with widths varying 
      from 5-400 mum are patterned on PA gels. Normal fibroblasts (MKF) are cultured on 
      the gel surfaces. The cell attachment and proliferation are confined within these 
      patterns. The method avoids the use of any toxic chemistry often used to pattern 
      different proteins on gel surfaces.
FAU - Tang, Xin
AU  - Tang X
AD  - Department of Mechanical Science and Engineering, College of Engineering, 
      University of Illinois at Urbana-Champaign, Urbana, Illinois, USA, 61801.
FAU - Ali, M Yakut
AU  - Ali MY
FAU - Saif, M Taher A
AU  - Saif MT
LA  - eng
GR  - R25 CA154015/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Soft Matter
JT  - Soft matter
JID - 101295070
PMC - PMC3447741
MID - NIHMS382126
EDAT- 2012/09/25 06:00
MHDA- 2012/09/25 06:01
PMCR- 2013/07/21
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2012/09/25 06:01 [medline]
PHST- 2013/07/21 00:00 [pmc-release]
AID - 10.1039/C2SM25533B [doi]
PST - ppublish
SO  - Soft Matter. 2012 Jul 21;8(27):7197-7206. doi: 10.1039/C2SM25533B.