PMID- 23002443
OWN - NLM
STAT- MEDLINE
DCOM- 20130109
LR  - 20211021
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 189
IP  - 9
DP  - 2012 Nov 1
TI  - Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.
PG  - 4379-86
LID - 10.4049/jimmunol.1201176 [doi]
AB  - B lymphopoiesis declines with age, and in rabbits this occurs by 8 wk of age. We 
      found that CFU fibroblasts (CFU-Fs) in the bone marrow (BM) decrease 10-fold by a 
      few weeks of age and that the CFU-Fs preferentially differentiate into adipocytes 
      instead of osteoblasts. BM becomes filled with fat spaces during this time, 
      making rabbit a unique model to study the effects of accelerated fat accumulation 
      on B lymphopoiesis. We show that adipocytes of both rabbit and human secrete a 
      soluble factor(s) that inhibits B lymphopoiesis, and we tested if this inhibition 
      was due to effects on the BM stroma or hematopoietic progenitors. Pretreatment of 
      BM mononuclear cells with adipocyte conditioned medium dramatically inhibited 
      their differentiation into proB cells in cocultures with OP9 stromal cells. In 
      contrast, pretreatment of OP9 stromal cells with adipocyte conditioned medium had 
      no effect on B lymphopoiesis. Using human hematopoietic stem cells, we show that 
      inhibition by the adipocyte-derived factor occurred at the common lymphoid 
      progenitor to preproB cell stage. We propose that the age-related decline in B 
      lymphopoiesis is due to a decrease in CFU-Fs, an increase in adipocytes, and an 
      adipocyte-derived factor that blocks B lymphopoiesis at the common lymphoid 
      progenitor to preproB cell stage.
FAU - Bilwani, Fareena A
AU  - Bilwani FA
AD  - Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 
      60153, USA.
FAU - Knight, Katherine L
AU  - Knight KL
LA  - eng
GR  - R01 AI068390/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120921
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (FABP4 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Growth Inhibitors)
SB  - IM
MH  - Adipocytes/*physiology
MH  - Animals
MH  - B-Lymphocyte Subsets/*cytology/*immunology/metabolism
MH  - Cell Line
MH  - Fatty Acid-Binding Proteins/*physiology
MH  - Fibroblasts/cytology/immunology/metabolism
MH  - Growth Inhibitors/*physiology
MH  - Humans
MH  - Lymphopoiesis/*immunology
MH  - Osteoblasts/cytology/immunology/metabolism
MH  - Rabbits
MH  - Stem Cells/cytology/immunology/metabolism/physiology
PMC - PMC3483032
MID - NIHMS403412
COIS- DISCLOUSRE: The authors have no conflicting financial interests.
EDAT- 2012/09/25 06:00
MHDA- 2013/01/10 06:00
PMCR- 2013/11/01
CRDT- 2012/09/25 06:00
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/01/10 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - jimmunol.1201176 [pii]
AID - 10.4049/jimmunol.1201176 [doi]
PST - ppublish
SO  - J Immunol. 2012 Nov 1;189(9):4379-86. doi: 10.4049/jimmunol.1201176. Epub 2012 
      Sep 21.