PMID- 23006534
OWN - NLM
STAT- MEDLINE
DCOM- 20130401
LR  - 20240321
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 10
DP  - 2012 Sep 24
TI  - Effects of helium and air inhalation on the innate and early adaptive immune 
      system in healthy volunteers ex vivo.
PG  - 201
LID - 10.1186/1479-5876-10-201 [doi]
AB  - BACKGROUND: Helium inhalation protects myocardium, brain and endothelium against 
      ischemia/reperfusion injury in animals and humans, when applied according to 
      specific "conditioning" protocols. Before widespread use of this "conditioning" 
      agent in clinical practice, negative side effects have to be ruled out. We 
      investigated the effect of prolonged helium inhalation on the responsiveness of 
      the human immune response in whole blood ex vivo. METHODS: Male healthy 
      volunteers inhaled 30 minutes heliox (79%He/21%O(2)) or air in a cross over 
      design, with two weeks between measurements. Blood was withdrawn at T0 
      (baseline), T1 (25 min inhalation) and T2-T5 (1, 2, 6, 24 h after inhalation) and 
      incubated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), T-cell stimuli 
      anti-CD3/ anti-CD28 (TCS) or RPMI (as control) for 2, 4 and 24 hours or not 
      incubated (0 h). An additional group of six volunteers inhaled 60 minutes of 
      heliox or air, followed by blood incubation with LPS and RPMI. Tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), interleukin-8 
      (IL-8), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) was analyzed by cytometric 
      bead array. Statistical analysis was performed by the Wilcoxon test for matched 
      samples. RESULTS: Incubation with LPS, LTA or TCS significantly increased TNF-alpha, 
      IL-1beta, IL-6, IL-8, IFN-gamma and IL-2 in comparison to incubation with RPMI alone. 
      Thirty min of helium inhalation did not influence the amounts of TNF-alpha, IL-1beta, 
      IL-6, IL-8, IFN-gamma and IL-2 in comparison to air. Sixty min of helium inhalation 
      did not affect cytokine production after LPS stimulation. CONCLUSIONS: We 
      conclude that 79% helium inhalation does not affect the responsiveness of the 
      human immune system in healthy volunteers. TRIAL REGISTRATION: Dutch Trial 
      Register: http://www.trialregister.nl/ NTR2152.
FAU - Oei, Gezina T M L
AU  - Oei GT
AD  - Laboratory of Experimental Intensive Care and Anesthesiology, Academic Medical 
      Centre, University of Amsterdam, The Netherlands.
FAU - Smit, Kirsten F
AU  - Smit KF
FAU - vd Vondervoort, Djai
AU  - vd Vondervoort D
FAU - Brevoord, Daniel
AU  - Brevoord D
FAU - Hoogendijk, Arjan
AU  - Hoogendijk A
FAU - Wieland, Catharina W
AU  - Wieland CW
FAU - Hollmann, Markus W
AU  - Hollmann MW
FAU - Preckel, Benedikt
AU  - Preckel B
FAU - Weber, Nina C
AU  - Weber NC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120924
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Cytokines)
RN  - 206GF3GB41 (Helium)
SB  - IM
MH  - *Air
MH  - Cytokines/metabolism
MH  - Helium/*administration & dosage
MH  - Humans
MH  - *Immunity, Innate
MH  - Inhalation Exposure
MH  - Male
MH  - Reference Values
PMC - PMC3495766
EDAT- 2012/09/26 06:00
MHDA- 2013/04/02 06:00
PMCR- 2012/09/24
CRDT- 2012/09/26 06:00
PHST- 2012/08/06 00:00 [received]
PHST- 2012/09/19 00:00 [accepted]
PHST- 2012/09/26 06:00 [entrez]
PHST- 2012/09/26 06:00 [pubmed]
PHST- 2013/04/02 06:00 [medline]
PHST- 2012/09/24 00:00 [pmc-release]
AID - 1479-5876-10-201 [pii]
AID - 10.1186/1479-5876-10-201 [doi]
PST - epublish
SO  - J Transl Med. 2012 Sep 24;10:201. doi: 10.1186/1479-5876-10-201.