PMID- 23007683
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20240109
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 20
IP  - 6
DP  - 2012 Aug
TI  - An integrated cytogenetic and physical map reveals unevenly distributed 
      recombination spots along the papaya sex chromosomes.
PG  - 753-67
LID - 10.1007/s10577-012-9312-1 [doi]
AB  - Papaya is a model system for the study of sex chromosome evolution in plants. 
      However, the cytological structures of the papaya chromosomes remain largely 
      unknown and chromosomal features have not been linked with any genetic or genomic 
      data. We constructed a cytogenetic map of the papaya sex chromosome (chromosome 
      1) by hybridizing 16 microsatellite markers and 2 cytological feature-associated 
      markers on pachytene chromosomes using fluorescence in situ hybridization (FISH). 
      Except for three markers, the order of the markers was concordant to that of 
      marker loci along the linkage map. This discrepancy was likely caused by skewed 
      segregation in the highly heterochromatic or centromeric regions. The papaya sex 
      chromosome is largely euchromatic, its heterochromatin spans about 15 % of the Y 
      chromosome and is mostly restricted to the centromeric and pericentromeric 
      regions. Analysis of the recombination frequency along the papaya sex chromosome 
      revealed a complete suppression of recombination in the centromere and 
      pericentromere region and 60 % higher recombination rate in the long arm than in 
      the short arm. The uneven distribution of recombination events might be caused by 
      differences in sequence composition. Sequence analysis of 18 scaffolds in total 
      length of 15 Mb revealed higher gene density towards the telomeres and lower gene 
      density towards the centromere, and a relatively higher gene density in the long 
      arm than in the short arm. In an opposite trend, the centromeric and 
      pericentromeric region contained the highest repetitive sequences and the long 
      arm showed the lowest repetitive sequences. This cytogenetic map provides 
      essential information for evolutionary study of sex chromosomes in Caricaceae and 
      will facilitate the analysis of papaya sex chromosomes.
FAU - Wai, Ching Man
AU  - Wai CM
AD  - Department of Tropical Plant and Soil Sciences, University of Hawaii, Honolulu, 
      HI 96822, USA.
FAU - Moore, Paul H
AU  - Moore PH
FAU - Paull, Robert E
AU  - Paull RE
FAU - Ming, Ray
AU  - Ming R
FAU - Yu, Qingyi
AU  - Yu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120925
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Amplified Fragment Length Polymorphism Analysis
MH  - Base Sequence
MH  - Carica/*genetics
MH  - Cytogenetic Analysis
MH  - Genetic Markers/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Matrix Attachment Regions/genetics
MH  - Microsatellite Repeats/genetics
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping
MH  - Recombination, Genetic/*genetics
MH  - Sequence Analysis, DNA
MH  - Sex Chromosomes/*genetics
EDAT- 2012/09/26 06:00
MHDA- 2013/03/26 06:00
CRDT- 2012/09/26 06:00
PHST- 2012/06/11 00:00 [received]
PHST- 2012/08/22 00:00 [accepted]
PHST- 2012/08/21 00:00 [revised]
PHST- 2012/09/26 06:00 [entrez]
PHST- 2012/09/26 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
AID - 10.1007/s10577-012-9312-1 [doi]
PST - ppublish
SO  - Chromosome Res. 2012 Aug;20(6):753-67. doi: 10.1007/s10577-012-9312-1. Epub 2012 
      Sep 25.