PMID- 23007909
OWN - NLM
STAT- MEDLINE
DCOM- 20130109
LR  - 20191112
IS  - 1473-2262 (Electronic)
IS  - 1473-2262 (Linking)
VI  - 24
DP  - 2012 Sep 24
TI  - Multifunctional surfaces with biomimetic nanofibres and drug-eluting 
      micro-patterns for infection control and bone tissue formation.
PG  - 237-48
AB  - For long-term orthopaedic implants, the creation of a surface that is repulsive 
      to bacteria while adhesive to tissue cells represents a promising strategy to 
      control infection. To obtain such multifunctional surfaces, two possible 
      approaches were explored to incorporate a model antibiotic, rifampicin (Rf), into 
      the osteogenic polycaprolactone (PCL)/chitosan (CHS) biomimetic nanofibre meshes 
      by (1) blending Rf into the electrospinning solutions and then electrospinning 
      into nanofibres (i.e., Rf-incorporating fibres), or (2) depositing Rf-containing 
      poly(D,L-lactic-co-glycolic) acid (PLGA) micro-patterns onto the PCL/chitosan 
      nanofibre meshes via ink-jet printing (i.e., Rf-eluting micro-pattern/fibre). 
      Rapid release of Rf from both meshes was measured even though a relatively slower 
      release rate was obtained from the Rf-eluting micro-pattern ones. Antibacterial 
      assay with Staphylococcus epidermidis showed that both mesh surfaces could 
      effectively kill bacteria and prevent biofilm formation. However, only Rf-eluting 
      micro-pattern meshes favoured the attachment, spreading and metabolic activity of 
      preosteoblasts in the cell culture study. Furthermore, the Rf-eluting 
      micro-pattern meshes could better support the osteogenic differentiation of 
      preosteoblasts by up-regulating the gene expression of bone markers (type I 
      collagen and alkaline phosphatase). Clearly, compared to Rf-incorporating 
      nanofibre meshes, Rf-eluting micro-patterns could effectively prevent biofilm 
      formation without sacrificing the osteogenic properties of PCL/chitosan nanofibre 
      surfaces. This finding provides an innovative avenue to design multifunctional 
      surfaces for enhancing bone tissue formation while controlling infection.
FAU - Chen, X N
AU  - Chen XN
AD  - Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens 
      Institute of Technology, Hoboken, NJ 07030, USA.
FAU - Gu, Y X
AU  - Gu YX
FAU - Lee, J H
AU  - Lee JH
FAU - Lee, W Y
AU  - Lee WY
FAU - Wang, H J
AU  - Wang HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120924
PL  - United States
TA  - Eur Cell Mater
JT  - European cells & materials
JID - 100973416
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antibiotics, Antitubercular)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
RN  - 9012-76-4 (Chitosan)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*administration & dosage
MH  - Antibiotics, Antitubercular/administration & dosage/pharmacology
MH  - Bacterial Infections/prevention & control
MH  - Biofilms/drug effects
MH  - Biomimetic Materials/*chemistry
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chitosan/chemistry
MH  - Mice
MH  - Nanofibers/*chemistry
MH  - Osteoblasts/cytology
MH  - *Osteogenesis
MH  - Polyesters/chemistry
MH  - Rifampin/administration & dosage/pharmacology
MH  - Staphylococcus epidermidis/drug effects/physiology
EDAT- 2012/09/26 06:00
MHDA- 2013/01/10 06:00
CRDT- 2012/09/26 06:00
PHST- 2012/09/26 06:00 [entrez]
PHST- 2012/09/26 06:00 [pubmed]
PHST- 2013/01/10 06:00 [medline]
AID - vol024a17 [pii]
AID - 10.22203/ecm.v024a17 [doi]
PST - epublish
SO  - Eur Cell Mater. 2012 Sep 24;24:237-48. doi: 10.22203/ecm.v024a17.