PMID- 23010110
OWN - NLM
STAT- MEDLINE
DCOM- 20130509
LR  - 20121203
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 102
DP  - 2013 Feb 1
TI  - Immobilization of lipase on amino-cyclodextrin functionalized carbon nanotubes 
      for enzymatic catalysis at the ionic liquid-organic solvent interface.
PG  - 124-9
LID - S0927-7765(12)00453-5 [pii]
LID - 10.1016/j.colsurfb.2012.07.050 [doi]
AB  - Amino-cyclodextrin was covalently attached to multiwalled carbon nanotubes 
      (MWNTs). The functionalized MWNTs have a good dispersibility in water. The lipase 
      was adsorbed onto the functionalized MWNTs. The immobilized lipase was utilized 
      for the resolution of the model compound (R, S)-1-phenyl ethanol in heptane, the 
      ionic liquid [Bmim]PF(6) as well as the heptane/[Bmim]PF(6) mixture. In the 
      reaction media, the enzymatic activity of the immobilized lipase is much higher 
      than that of the native lipase. In comparison to the catalysis in the ionic 
      liquid and heptane, when using the mixture of heptane/[Bmim]PF(6) as the reaction 
      medium, the catalysis by the immobilized lipase at the heptane-ionic liquid 
      interface exhibited a higher catalysis activity. This is due to two aspects: the 
      continuous diffusion of substrate from the heptane phase to the ionic liquid 
      phase; the simultaneous extraction of product from the ionic liquid phase. In 
      addition, the interfacial enzymatic catalysis facilitates the reuse of the 
      immobilized lipase and the ionic liquid.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Li, Lili
AU  - Li L
AD  - Department of Biochemical Engineering, Beijing University of Chemical Technology, 
      Beijing, China.
FAU - Feng, Wei
AU  - Feng W
FAU - Pan, Kehou
AU  - Pan K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120814
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Cyclodextrins)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Ionic Liquids)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Solvents)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Cyclodextrins/*chemistry
MH  - Enzymes, Immobilized/*chemistry
MH  - Ionic Liquids/*chemistry
MH  - Lipase/*chemistry
MH  - Nanotubes, Carbon/*chemistry
MH  - Solvents/*chemistry
EDAT- 2012/09/27 06:00
MHDA- 2013/05/10 06:00
CRDT- 2012/09/27 06:00
PHST- 2012/05/21 00:00 [received]
PHST- 2012/07/01 00:00 [revised]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2012/09/27 06:00 [entrez]
PHST- 2012/09/27 06:00 [pubmed]
PHST- 2013/05/10 06:00 [medline]
AID - S0927-7765(12)00453-5 [pii]
AID - 10.1016/j.colsurfb.2012.07.050 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2013 Feb 1;102:124-9. doi: 
      10.1016/j.colsurfb.2012.07.050. Epub 2012 Aug 14.