PMID- 23010713
OWN - NLM
STAT- MEDLINE
DCOM- 20121204
LR  - 20120926
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 138
IP  - 4
DP  - 2012 Oct
TI  - Array-based karyotyping in plasma cell neoplasia after plasma cell enrichment 
      increases detection of genomic aberrations.
PG  - 579-89
AB  - The discovery of genomic abnormalities present in monoclonal plasma cells has 
      diagnostic, prognostic, and disease-monitoring implications in plasma cell 
      neoplasms (PCNs). However, technical and disease-related limitations hamper the 
      detection of these abnormalities using cytogenetic analysis or fluorescence in 
      situ hybridization (FISH). In this study, 28 bone marrow specimens with known 
      PCNs were examined for the presence of genomic abnormalities using microarray 
      analysis after plasma cell enrichment. Cytogenetic analysis was performed on 15 
      of 28 samples, revealing disease-related genomic aberrations in only 3 (20%) of 
      15 cases. FISH analysis was performed on enriched plasma cells and detected 
      aberrations in 84.6% of specimens while array comparative genomic hybridization 
      (aCGH) detected abnormalities in 89.3% of cases. Furthermore, aCGH revealed 
      additional abnormalities in 24 cases compared with FISH alone. We conclude that 
      aCGH after plasma cell enrichment, in combination with FISH, is a valuable 
      approach for routine clinical use in achieving a more complete genetic 
      characterization of patients with PCN.
FAU - Zehentner, Barbara K
AU  - Zehentner BK
AD  - Hematologics Inc, 3161 Elliott Ave, Ste 200, Seattle, WA 98121, USA. 
      Barbara@hematologics.com
FAU - Hartmann, Luise
AU  - Hartmann L
FAU - Johnson, Krystal R
AU  - Johnson KR
FAU - Stephenson, Christine F
AU  - Stephenson CF
FAU - Chapman, Douglas B
AU  - Chapman DB
FAU - de Baca, Monica E
AU  - de Baca ME
FAU - Wells, Denise A
AU  - Wells DA
FAU - Loken, Michael R
AU  - Loken MR
FAU - Tirtorahardjo, Budi
AU  - Tirtorahardjo B
FAU - Gunn, Shelly R
AU  - Gunn SR
FAU - Lim, Lony
AU  - Lim L
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Cells
MH  - Cell Separation
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization/*methods
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasms, Plasma Cell/diagnosis/*genetics
MH  - Plasma Cells/*pathology
EDAT- 2012/09/27 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/09/27 06:00
PHST- 2012/09/27 06:00 [entrez]
PHST- 2012/09/27 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 138/4/579 [pii]
AID - 10.1309/AJCPKW31BAIMVGST [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2012 Oct;138(4):579-89. doi: 10.1309/AJCPKW31BAIMVGST.