PMID- 23012486
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120928
LR  - 20211021
IS  - 1347-5800 (Electronic)
IS  - 0044-5991 (Print)
IS  - 0044-5991 (Linking)
VI  - 45
IP  - 4
DP  - 2012 Aug 30
TI  - Expression and localization of brain-derived neurotrophic factor (BDNF) mRNA and 
      protein in human submandibular gland.
PG  - 211-8
AB  - Brain-derived neurotrophic factor (BDNF) promotes cell survival and 
      differentiation in the central and peripheral nervous systems. Previously, we 
      reported that BDNF is produced by salivary glands under acute immobilization 
      stress in rats. However, expression of BDNF is poorly understood in humans, 
      although salivary gland localization of BDNF in rodents has been demonstrated. In 
      the present study, we investigated the expression and localization of BDNF in the 
      human submandibular gland (HSG) using reverse transcription-polymerase chain 
      reaction, western blot analysis, in situ hybridization (ISH), 
      immunohistochemistry (IHC), and ELISA. BDNF was consistently localized in HSG 
      serous and ductal cells, as detected by ISH and IHC, with reactivity being 
      stronger in serous cells. In addition, immunoreactivity for BDNF was observed in 
      the saliva matrix of ductal cavities. Western blotting detected one significant 
      immunoreactive 14 kDa band in the HSG and saliva. Immunoreactivities for salivary 
      BDNF measured by ELISA in humans were 40.76+/-4.83 pg/mL and 52.64+/-8.42 pg/mL, in 
      men and women, respectively. Although salivary BDNF concentrations in females 
      tended to be higher than in males, the concentrations were not significantly 
      different. In conclusion, human salivary BDNF may originate from salivary glands, 
      as the HSG appears to produce BDNF.
FAU - Saruta, Juri
AU  - Saruta J
AD  - Department of Maxillofacial Diagnostic Science, Division of Pathology, Kanagawa 
      Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan ; Research 
      Institute of Salivary Gland and Health Medicine, Kanagawa Dental College, 82 
      Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
FAU - Fujino, Kazuhiro
AU  - Fujino K
FAU - To, Masahiro
AU  - To M
FAU - Tsukinoki, Keiichi
AU  - Tsukinoki K
LA  - eng
PT  - Journal Article
DEP - 20120630
PL  - Japan
TA  - Acta Histochem Cytochem
JT  - Acta histochemica et cytochemica
JID - 0147110
PMC - PMC3445760
OTO - NOTNLM
OT  - brain-derived neurotrophic factor (BDNF)
OT  - human
OT  - saliva
OT  - submandibular gland
EDAT- 2012/09/27 06:00
MHDA- 2012/09/27 06:01
PMCR- 2012/08/30
CRDT- 2012/09/27 06:00
PHST- 2012/01/28 00:00 [received]
PHST- 2012/04/09 00:00 [accepted]
PHST- 2012/09/27 06:00 [entrez]
PHST- 2012/09/27 06:00 [pubmed]
PHST- 2012/09/27 06:01 [medline]
PHST- 2012/08/30 00:00 [pmc-release]
AID - AHC12005 [pii]
AID - 10.1267/ahc.12005 [doi]
PST - ppublish
SO  - Acta Histochem Cytochem. 2012 Aug 30;45(4):211-8. doi: 10.1267/ahc.12005. Epub 
      2012 Jun 30.