PMID- 23015127
OWN - NLM
STAT- MEDLINE
DCOM- 20130304
LR  - 20120927
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 55
IP  - 9
DP  - 2012 Sep
TI  - Evolution of quasispecies diversity for porcine reproductive and respiratory 
      syndrome virus under antibody selective pressure.
PG  - 788-92
AB  - To study the quasispecies diversity of porcine reproductive and respiratory 
      syndrome virus (PRRSV), open reading frame 5 (ORF5) of strain SD0612 was 
      amplified and cloned. Sixty clones of ORF5 were sequenced and analyzed with 
      DNAStar software. Nucleic acid sequence homology was 97.7%-100%, with 78 
      mutations observed. Among these 60 clones, the sequences of 17 clones were 
      identical and recognized as the dominant quasispecies of strain SD0612. Evolution 
      of SD0612 quasispecies diversity under antibody selective pressure was also 
      studied. SD0612 was passed continuously in the Marc-145 cell line over 40 
      passages in 6 independent lineages. SD0612 antiserum was not added to lineage A, 
      B, and C cultures; however, antiserum was added to culture medium for lineages D, 
      E, and F. PRRSV ORF5 was then amplified, cloned, and sequenced from each of the 6 
      lineages, designated as A40-F40. F40 was further passed in Marc-145 cells using 6 
      independent lineages with or without F40 antiserum for another 40 passages. ORF5 
      from the 6 newly-derived virus lineages, which we designated as a40-f40, were 
      amplified, cloned and sequenced. The proportion of dominant quasispecies 
      increased with passage number in cell cultures supplemented with antibodies, but 
      decreased when antibodies were lacking. Our work has demonstrated a diversity of 
      quasispecies for ORF5 in PRRSV SD0612. Antibody selective pressure was able to 
      significantly influence quasispecies diversity and promote a dominant 
      quasispecies that was able to evade immune reactions.
FAU - Zhao, Peng
AU  - Zhao P
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, 
      China.
FAU - Ma, ChengTai
AU  - Ma C
FAU - Dong, Xuan
AU  - Dong X
FAU - Cui, ZhiZhong
AU  - Cui Z
LA  - eng
PT  - Journal Article
DEP - 20120927
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology/*pharmacology
MH  - Cell Line
MH  - Evolution, Molecular
MH  - Genetic Variation/*drug effects/genetics
MH  - Immune Sera/immunology/pharmacology
MH  - Mutation
MH  - Open Reading Frames/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Swine/virology
MH  - Virus Replication/*drug effects/genetics
EDAT- 2012/09/28 06:00
MHDA- 2013/03/05 06:00
CRDT- 2012/09/28 06:00
PHST- 2012/05/13 00:00 [received]
PHST- 2012/06/29 00:00 [accepted]
PHST- 2012/09/28 06:00 [entrez]
PHST- 2012/09/28 06:00 [pubmed]
PHST- 2013/03/05 06:00 [medline]
AID - 10.1007/s11427-012-4364-1 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2012 Sep;55(9):788-92. doi: 10.1007/s11427-012-4364-1. Epub 
      2012 Sep 27.