PMID- 23016293
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 6
DP  - 2012 Jun
TI  - [Determination of carbon-centred radicals in mainstream cigarette smoke using 
      spin-labelled fluorophore].
PG  - 590-5
AB  - A method of the determination of the carbon-centred radicals in mainstream 
      cigarette smoke using a spin-labelled fluorophore, 4-((9-acridinecarbonyl) 
      amino)-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO-9-AC), as a fluorescent probe 
      is presented. After being producted by smoking in International Organization for 
      Standardization (ISO) mode, the carbon-centred radicals in mainstream cigarette 
      smoke were trapped by TEMPO-9-AC, a carbon-centred radical probe with a low 
      fluorescence intensity. Then the latter was transformed to a stable diamagnetic 
      o-alkoxyamine, a high-fluorescence compound. Finally, high performance liquid 
      chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to demonstrate the 
      structures of the carbon-centred radicals, and high performance liquid 
      chromatography with a fluorescence detector (HPLC-FLD) was used to determine the 
      concentration of the carbon-centred radicals. The results showed that the 10 
      carbon-centred radicals were detected in the mainstream cigarette smoke, and the 
      total carbon-centred radicals concentrations for 1R5F, 3R4F, CM6, and two 
      Virginia type cigarettes, were 52.5 nmol/cig, 214.6 nmol/cig, 424.1 nmol/cig, 
      68.6 nmol/cig, and 334.2 nmol/cig, respectively; and there was positive relation 
      between the concentrations of the total amount of carbon-centred radicals and the 
      tar amounts in the mainstream cigarette smoke. The detection limit was 0.318 
      nmol/cig, and the relative standard deviations (RSDs) ranged from 3.5% to 9.7%. 
      This method is suitable for the determination of the carbon-centred radicals in 
      the mainstream cigarette smoke.
FAU - Bian, Zhaoyang
AU  - Bian Z
AD  - China National Tobacco Quality Supervision &Test Center, Zhengzhou 450001, China. 
      zybian@126.com
FAU - Tang, Gangling
AU  - Tang G
FAU - Yang, Fei
AU  - Yang F
FAU - Pang, Yongqiang
AU  - Pang Y
FAU - Zhang, Hongfei
AU  - Zhang H
FAU - Hu, Qingyuan
AU  - Hu Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
EDAT- 2012/09/29 06:00
MHDA- 2012/09/29 06:01
CRDT- 2012/09/29 06:00
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2012/09/29 06:01 [medline]
AID - 10.3724/sp.j.1123.2012.01007 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jun;30(6):590-5. doi: 10.3724/sp.j.1123.2012.01007.