PMID- 23016296
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 6
DP  - 2012 Jun
TI  - [Determination of homocysteine in plasma by precolumn derivatization-high 
      performance liquid chromatography with fluorescence detection].
PG  - 613-7
AB  - A precolumn derivatization-high performance liquid chromatographic method for the 
      determination of homocysteine (Hcy) in plasma was established. Tris 
      (2-carboxyethyl) phosphine hydrochloride (TCEP) and N-(1-pyrenyl) maleimide (NPM) 
      were used as the reduced reagent and derivatization reagent, respectively. The 
      separation was carried out on an Agilent Hypersil C-18 column (250 mm x 4.0 mm, 5 
      microm) in gradient elution mode. The mobile phase consisted of A (15 mmol/L 
      sodium acetate solution), B (acetonitrile) and C (300 mL water containing 1 mL 
      acetic acid and 1 mL phosphoric acid). The eluate was monitored by the 
      fluorescence detector at an excitation wavelength of 330 nm and an emission 
      wavelength of 380 nm. The mean recovery of Hcy was (102.08 +/- 4.94)%. The linear 
      range was from 0.500 micromol/L to 100 micromol/L, with a detection limit of 
      0.016 micromol/L. The intra-day and inter-day relative standard deviations (RSDs) 
      for Hcy were less than 5%. Seven plasma samples of patients with hypertension and 
      seven plasma samples of healthy controls were tested, and the results 
      demonstrated that the Hcy in the plasma from the hypertension group was 
      significantly different from that of the control group (p < 0.05). The developed 
      method is simple, fast, accurate, and suitable for clinical measurement.
FAU - Tang, Xiufang
AU  - Tang X
AD  - College of Laboratory Medicine, Chongqing Medical University, Key Laboratory of 
      Clinical Laboratory Diagnostics of Ministry of Education, Chongqing 400016, 
      China.
FAU - Zhen, Qianna
AU  - Zhen Q
FAU - Fan, Zimian
AU  - Fan Z
FAU - Feng, Chengya
AU  - Feng C
FAU - Ding, Min
AU  - Ding M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0LVT1QZ0BA (Homocysteine)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid/*instrumentation/*methods
MH  - Female
MH  - Homocysteine/*blood
MH  - Humans
MH  - Hypertension/blood
MH  - Male
MH  - Middle Aged
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2012/09/29 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/09/29 06:00
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.03026 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jun;30(6):613-7. doi: 10.3724/sp.j.1123.2012.03026.