PMID- 23016301
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 6
DP  - 2012 Jun
TI  - [Simultaneous determination of 6 antibiotics and metronidazole in acne removal 
      products bt high performance liquid chromatography].
PG  - 641-6
AB  - An analytical method for the simultaneous determination of 6 antibiotics 
      (minocycline, oxytetracycline, tetracycline hydrochloride, chlorotetracycline 
      hydrochloride, doxycycline hydrochloride and chloramphenicol) and metronidazole 
      in acne removal products of cosmetic was established using high performance 
      liquid chromatography (HPLC). The drugs in the sample were extracted with 
      methanol. The separation was performed on an Agilent ZORBAX SB-C18 column (250 mm 
      x 4.6 mm, 5 microm) at 20 degrees C with methanol, acetonitrile and 0.002 mol/L 
      oxalic acid solution as mobile phases with gradient elution at a flow rate of 0.8 
      mL/min. The detection was performed by a diode array detector (DAD) at 268 nm. 
      The injection volume was 10 microL. The quantification was performed by external 
      standard method. The calibration curves showed good linearity within the range of 
      1 - 30 mg/L with the correlation coefficients no less than 0.997 0. The detection 
      limits were in the range of 1.1 - 1.2 microg/g. The recoveries were between 91.9% 
      and 107.7% in three spiked levels of 5, 10 and 20 mg/L with the relative standard 
      deviations (RSDs) of 0.13% - 1.74%. The method was used in the analysis of acne 
      removal products, and metronidazole was found in 15% of the total test samples. 
      The method is rapid, sensitive, accurate, effective in separation, and can be 
      used in the determination of the six antibiotics and metronidazole in acne 
      removal products.
FAU - Lu, Jun
AU  - Lu J
AD  - Liaoning Institute for Food and Drug Control, Shenyang 110023, China. 
      zsc671225@163.com
FAU - Pang, Yanjun
AU  - Pang Y
FAU - Li, Yanbo
AU  - Li Y
FAU - Wang, Chao
AU  - Wang C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Cosmetics)
RN  - 140QMO216E (Metronidazole)
RN  - F8VB5M810T (Tetracycline)
RN  - FYY3R43WGO (Minocycline)
RN  - X20I9EN955 (Oxytetracycline)
SB  - IM
MH  - Acne Vulgaris/*therapy
MH  - Anti-Bacterial Agents/*analysis
MH  - Anti-Infective Agents/analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cosmetics/*chemistry
MH  - Humans
MH  - Metronidazole/*analysis
MH  - Minocycline/analysis
MH  - Oxytetracycline/analysis
MH  - Tetracycline/analysis
EDAT- 2012/09/29 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/09/29 06:00
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.02024 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jun;30(6):641-6. doi: 10.3724/sp.j.1123.2012.02024.