PMID- 23016400
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR  - 20120928
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 29
IP  - 4
DP  - 2012 Aug
TI  - [The effects of PEMF on the activation of human monocytes].
PG  - 604-8
AB  - The aim of the present study is to investigate the effect of pulsed 
      electromagnetic field (PEMF) on the activation of human monocytes (THP-1). 
      Cultured THP-1 cells were exposed to PEMF stimulation with radiation of 32Hz or 
      64Hz respectively, using sinusoidal wave, and 1mT, twice a day, 30 minutes each 
      time, with an interval of 8 hours, for 3 days. Those with 0Hz stimulation served 
      as the controls. Monocytes activation was monitored by measuring both the release 
      of monocyte chemoattractant protein-1 (MCP-1) from monocytes and their adhesion 
      to monolayers of human umbilical vein endothelial cells (HUVECs). The adhesion of 
      THP-1 cells to HUVECs was evaluated by cell counting method. The secretion of 
      MCP-1 from THP-1 cells was detected by ELISA and MCP-1 mRNA expression was 
      assessed by real time quantitative RT-PCR. The data showed that exposure to PEMF 
      with above parameters could significantly inhibit the adhesion of THP-1 cells to 
      HUVECs and decrease the MCP-1 mRNA and protein expression. The results 
      demonstrated that exposure to PEMF of 1mT, 32Hz or 64Hz for 3 days could 
      significantly inhibit the activation of THP-1 cells.
FAU - Chen, Xiaoying
AU  - Chen X
AD  - Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan University, 
      Chengdu 610041, China.
FAU - Han, Xiaoyu
AU  - Han X
FAU - Wang, Qian
AU  - Wang Q
FAU - Wu, Wenchao
AU  - Wu W
FAU - Liu, Xiaojing
AU  - Liu X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - *Electromagnetic Fields
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Monocytes/*cytology
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2012/09/29 06:00
MHDA- 2013/11/01 06:00
CRDT- 2012/09/29 06:00
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2012 Aug;29(4):604-8.