PMID- 23018230
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20220310
IS  - 1807-3107 (Electronic)
IS  - 1806-8324 (Linking)
VI  - 26
IP  - 5
DP  - 2012 Sep-Oct
TI  - Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm 
      of pregnant women.
PG  - 443-9
LID - S1806-83242012000500011 [pii]
AB  - The aim of this study was to use the fluorescence in situ hybridization (FISH) 
      technique to test the hypothesis of qualitative and quantitative differences of 8 
      periodontopathogens between pregnant and non-pregnant women. This cross-sectional 
      study included 20 pregnant women in their second trimester of pregnancy and 20 
      non-pregnant women. Probing depth, bleeding on probing, clinical attachment 
      level, and presence of calculus were recorded. Subgingival plaque samples were 
      collected and the FISH technique identified the presence and numbers of 
      Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter 
      rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, 
      Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was 
      applied to compare the data between the two groups. The mean age, ethnicity, 
      marital status, education, and economic level in both groups were similar. The 
      clinical parameters showed no significant differences between pregnant and 
      non-pregnant women. The numbers of subgingival periodontopathogens were not found 
      to be significantly different between groups, despite the higher mean counts of 
      P. intermedia in pregnant women. Colonization patterns of the different bacteria 
      most commonly associated with periodontal disease were not different in the 
      subgingival plaque of pregnant and non-pregnant women.
FAU - Machado, Fernanda Campos
AU  - Machado FC
AD  - School of Medicine, Univ Federal de Juiz de Fora, MG, Brazil. 
      fercampo@terra.com.br
FAU - Cesar, Dioneia Evangelista
AU  - Cesar DE
FAU - Assis, Amanda Vervloet Dutra Agostinho
AU  - Assis AV
FAU - Diniz, Claudio Galuppo
AU  - Diniz CG
FAU - Ribeiro, Rosangela Almeida
AU  - Ribeiro RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz Oral Res
JT  - Brazilian oral research
JID - 101307187
SB  - IM
MH  - Adult
MH  - Bacteria/*growth & development
MH  - Bacterial Load/*methods
MH  - Biofilms/*growth & development
MH  - Cross-Sectional Studies
MH  - Dental Health Surveys
MH  - Female
MH  - Gingiva/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Periodontal Diseases/microbiology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/microbiology
MH  - Statistics, Nonparametric
MH  - Young Adult
EDAT- 2012/09/29 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/09/29 06:00
PHST- 2012/05/15 00:00 [received]
PHST- 2012/06/05 00:00 [accepted]
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - S1806-83242012000500011 [pii]
AID - 10.1590/s1806-83242012000500011 [doi]
PST - ppublish
SO  - Braz Oral Res. 2012 Sep-Oct;26(5):443-9. doi: 10.1590/s1806-83242012000500011.