PMID- 23018248
OWN - NLM
STAT- MEDLINE
DCOM- 20130314
LR  - 20170123
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 27
IP  - 11
DP  - 2012 Nov
TI  - Expression of matricellular proteins in human uterine leiomyomas and normal 
      myometrium.
PG  - 1495-502
LID - 10.14670/HH-27.1495 [doi]
AB  - Growth of human leiomyomas can probably be initiated as a response to injury, in 
      a way similar to the development of keloids. Among many bioactive molecules, 
      which are implicated in tissue repair, a pivotal role is attributed to 
      matricellular proteins. The aim of the current study was to evaluate the 
      immunohistochemical expression of tenascin-C (TNC), thrombospondin-1 (TSP-1), 
      SPARC/osteonectin and tenascin-X (TNX) in human uterine leiomyomas and normal 
      myometrium. Immunostaining was performed on 33 pairs of paraffin-fixed sections 
      and 9 cell-lines derived from uterine leiomyomas and normal myometrium. Fifteen 
      (45.5%) leiomyomas investigated were positive for TNC, whereas all normal 
      myometrial samples were immunonegative (chi(2)=19.41; p<0.001). Immunostaining for 
      TSP-1 was observed in 20 (60.6%) uterine fibroids and in 12 (36.4%) control 
      samples (chi(2)=3.88; p<0.05). The expression of SPARC/osteonectin protein was more 
      frequently found in leiomyomas than in normal myometrium, but this difference was 
      not significant. Apart from one fibroid culture and one myometrial culture, all 
      the others revealed strong TNC immunostaining. Expression of TSP-1 and 
      SPARC/osteonectin was weak to moderate in all established cell-lines. None of the 
      tissues or cell lines investigated showed positive staining for TNX. In 
      conclusion, TSP-1 and TNC are likely to play important roles in the pathogenesis 
      of uterine leiomyomas, presumably affecting cell proliferation and/or 
      extracellular matrix deposition.
FAU - Bogusiewicz, Michal
AU  - Bogusiewicz M
AD  - 2nd Department of Gynecology, Medical University of Lublin, Lublin, Poland.
FAU - Semczuk, Andrzej
AU  - Semczuk A
FAU - Juszczak, Malgorzata
AU  - Juszczak M
FAU - Langner, Ewa
AU  - Langner E
FAU - Walczak, Katarzyna
AU  - Walczak K
FAU - Rzeski, Wojciech
AU  - Rzeski W
FAU - Tomaszewski, Jacek
AU  - Tomaszewski J
FAU - Rechberger, Tomasz
AU  - Rechberger T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Osteonectin)
RN  - 0 (Tenascin)
RN  - 0 (Thrombospondin 1)
RN  - 0 (tenascin X)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/*metabolism
MH  - Female
MH  - Humans
MH  - Leiomyoma/*metabolism/pathology
MH  - Middle Aged
MH  - Myometrium/*metabolism/pathology
MH  - Osteonectin/metabolism
MH  - Tenascin/metabolism
MH  - Thrombospondin 1/metabolism
MH  - Uterine Neoplasms/*metabolism/pathology
EDAT- 2012/09/29 06:00
MHDA- 2013/03/15 06:00
CRDT- 2012/09/29 06:00
PHST- 2012/09/29 06:00 [entrez]
PHST- 2012/09/29 06:00 [pubmed]
PHST- 2013/03/15 06:00 [medline]
AID - 10.14670/HH-27.1495 [doi]
PST - ppublish
SO  - Histol Histopathol. 2012 Nov;27(11):1495-502. doi: 10.14670/HH-27.1495.