PMID- 23021259
OWN - NLM
STAT- MEDLINE
DCOM- 20140212
LR  - 20181203
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 25
IP  - 6
DP  - 2013
TI  - Effects of FSH on the expression of receptors for oocyte-secreted factors and 
      members of the EGF-like family during in vitro maturation in cattle.
PG  - 890-9
LID - 10.1071/RD12125 [doi]
AB  - FSH induces expansion of bovine cumulus-oocyte complexes (COCs) in cattle, which 
      can be enhanced by oocyte-secreted factors (OSFs). In this study it was 
      hypothesised that FSH stimulates COC expansion in part from direct stimulation of 
      the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin 
      (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or 
      their receptors in cumulus cells. Bovine COCs were cultured in defined medium 
      with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused 
      cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- 
      and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest 
      stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 
      15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression 
      of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More 
      interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors 
      for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH 
      also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. 
      Based on dose-response studies, FGFR3C was the receptor most sensitive to the 
      influence of FSH. This study demonstrates that FSH stimulates the expression of 
      EGF-like factors in bovine cumulus cells, and provides evidence that FSH 
      differently regulates the expression of distinct receptors for OSFs in cumulus 
      cells.
FAU - Caixeta, Ester Siqueira
AU  - Caixeta ES
AD  - Departamento de Farmacologia, Instituto de Biociencias, Universidade Estadual 
      Paulista Julio de Mesquita Filho, Rubiao Junior, Botucatu, Sao Paulo 18618-970, 
      Brazil.
FAU - Machado, Mariana Fernandes
AU  - Machado MF
FAU - Ripamonte, Paula
AU  - Ripamonte P
FAU - Price, Christopher
AU  - Price C
FAU - Buratini, Jose
AU  - Buratini J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Amphiregulin)
RN  - 0 (Betacellulin)
RN  - 0 (Epiregulin)
RN  - 0 (Fertility Agents, Female)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 0 (Protein Isoforms)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2)
RN  - EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I)
SB  - IM
MH  - Abattoirs
MH  - Amphiregulin
MH  - Animals
MH  - Betacellulin
MH  - Bone Morphogenetic Protein Receptors, Type I/biosynthesis/genetics/metabolism
MH  - Cattle/*physiology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques/veterinary
MH  - Cumulus Cells/cytology/*drug effects/metabolism
MH  - Epidermal Growth Factor/biosynthesis/genetics/*metabolism
MH  - Epiregulin
MH  - Female
MH  - Fertility Agents, Female/*pharmacology
MH  - Follicle Stimulating Hormone/*pharmacology
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Glycoproteins/biosynthesis/genetics/metabolism
MH  - In Vitro Oocyte Maturation Techniques/*veterinary
MH  - Intercellular Signaling Peptides and Proteins/biosynthesis/genetics/metabolism
MH  - Ligands
MH  - Oocytes/cytology/*drug effects/metabolism
MH  - Protein Isoforms/biosynthesis/genetics/metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 2/biosynthesis/genetics/metabolism
MH  - Sus scrofa
EDAT- 2012/10/02 06:00
MHDA- 2014/02/13 06:00
CRDT- 2012/10/02 06:00
PHST- 2012/04/18 00:00 [received]
PHST- 2012/07/25 00:00 [accepted]
PHST- 2012/10/02 06:00 [entrez]
PHST- 2012/10/02 06:00 [pubmed]
PHST- 2014/02/13 06:00 [medline]
AID - RD12125 [pii]
AID - 10.1071/RD12125 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2013;25(6):890-9. doi: 10.1071/RD12125.