PMID- 23023090
OWN - NLM
STAT- MEDLINE
DCOM- 20130411
LR  - 20210103
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 387
IP  - 1-2
DP  - 2013 Jan 31
TI  - A novel assay for detecting virus-specific antibodies triggering activation of 
      Fcgamma receptors.
PG  - 21-35
LID - S0022-1759(12)00288-8 [pii]
LID - 10.1016/j.jim.2012.09.006 [doi]
AB  - IgG responses are crucial in antiviral defence and instrumental for the 
      serodiagnosis of infections. Fcgamma receptors (FcgammaRs), which recognize the Fc-part 
      of IgG, differ regarding their IgG binding affinity, IgG subclass preference, 
      cellular expression profile and pathogen elimination mechanisms elicited upon 
      activation. Assessing their activation in vitro is of fundamental importance, but 
      technically difficult. Therefore, a novel assay for measuring antiviral IgG 
      antibodies triggering activation of individual host Fcgamma receptors was 
      established. The assay comprises the co-cultivation of virus-infected target 
      cells with immune IgG antibodies and mouse BW5147 hybridoma cells stably 
      expressing chimeric FcgammaR-CD3zeta chain molecules consisting of the extracellular 
      domain of human FcgammaRIIIA, FcgammaRIIA or FcgammaRI, respectively, fused to the 
      transmembrane and intracellular domains of the mouse CD3zeta chain. Triggering of 
      the chimeric FcgammaR receptors by immune complexes formed on the surface of 
      IgG-opsonized virus-infected target cells resulted in FcgammaR activation leading to 
      IL-2 secretion by BW5147 cells, which was quantified as a surrogate marker in an 
      ELISA. Target cells infected with various human pathogenic viruses including 
      herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV), human 
      cytomegalovirus (HCMV), measles virus (MV), and respiratory syncytial virus (RSV) 
      or displaying human immunodeficiency virus-1 (HIV-1) gp120 evoke dose-dependent 
      IgG responses demonstrating the universal applicability of the assay. Taken 
      together, a new reliable and simple tool for measuring antibodies triggering 
      activation of Fcgamma receptors was established. This assay will be instrumental for 
      defining novel correlates of IgG immunity and the design of new therapeutic IgGs.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Corrales-Aguilar, Eugenia
AU  - Corrales-Aguilar E
AD  - Institute for Virology, Heinrich-Heine-University Dusseldorf, Universitatsstrasse 
      1, 40225 Dusseldorf, Germany.
FAU - Trilling, Mirko
AU  - Trilling M
FAU - Reinhard, Henrike
AU  - Reinhard H
FAU - Merce-Maldonado, Eva
AU  - Merce-Maldonado E
FAU - Widera, Marek
AU  - Widera M
FAU - Schaal, Heiner
AU  - Schaal H
FAU - Zimmermann, Albert
AU  - Zimmermann A
FAU - Mandelboim, Ofer
AU  - Mandelboim O
FAU - Hengel, Hartmut
AU  - Hengel H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120927
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies, Viral)
RN  - 0 (CD3 Complex)
RN  - 0 (CD3 antigen, zeta chain)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-2)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology/metabolism
MH  - CD3 Complex/genetics/immunology/metabolism
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - Cell Line, Tumor
MH  - Chlorocebus aethiops
MH  - Coculture Techniques
MH  - Cytomegalovirus/immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - HEK293 Cells
MH  - Herpesvirus 1, Human/immunology
MH  - Herpesvirus 4, Human/immunology
MH  - Humans
MH  - Hybridomas
MH  - Immunoglobulin G/immunology/metabolism
MH  - Interleukin-2/immunology/metabolism/pharmacology
MH  - Killer Cells, Natural/drug effects/immunology/metabolism
MH  - Mice
MH  - Protein Binding/immunology
MH  - Receptors, IgG/genetics/*immunology/metabolism
MH  - Recombinant Fusion Proteins/genetics/immunology/metabolism
MH  - U937 Cells
MH  - Vero Cells
MH  - Viruses/*immunology
EDAT- 2012/10/02 06:00
MHDA- 2013/04/12 06:00
CRDT- 2012/10/02 06:00
PHST- 2012/06/10 00:00 [received]
PHST- 2012/08/12 00:00 [revised]
PHST- 2012/09/19 00:00 [accepted]
PHST- 2012/10/02 06:00 [entrez]
PHST- 2012/10/02 06:00 [pubmed]
PHST- 2013/04/12 06:00 [medline]
AID - S0022-1759(12)00288-8 [pii]
AID - 10.1016/j.jim.2012.09.006 [doi]
PST - ppublish
SO  - J Immunol Methods. 2013 Jan 31;387(1-2):21-35. doi: 10.1016/j.jim.2012.09.006. 
      Epub 2012 Sep 27.