PMID- 23024038
OWN - NLM
STAT- MEDLINE
DCOM- 20130520
LR  - 20220310
IS  - 1099-0496 (Electronic)
IS  - 1099-0496 (Linking)
VI  - 47
IP  - 12
DP  - 2012 Dec
TI  - Nasal and bronchial airway epithelial cell mediator release in children.
PG  - 1215-25
LID - 10.1002/ppul.22672 [doi]
AB  - OBJECTIVE: The present study was designed to test the hypothesis that airway 
      epithelial cell (AEC) mediator release is similar in upper and lower airway AEC 
      in children. METHODS: Nasal and bronchial AEC were collected by brushings from 
      children scheduled for general anesthetic. AEC release of the following mediators 
      was measured: interleukin (IL)-6, IL-8, Granulocyte Colony Stimulating Factor 
      (G-CSF), regulated on activation, normal T-cell expressed and secreted (RANTES), 
      monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor 
      (VEGF), matrix metallo proteinase (MMP)-9, and tissue inhibitor of 
      metalloproteinases (TIMP)-1. RESULTS: AEC were cultured in 34 children, mean age 
      7.3 years. Release of IL-6, IL-8, and G-CSF was significantly higher in nasal 
      compared with bronchial AEC but nasal and bronchial AEC release of other 
      mediators was not significantly different. Treatment of AEC with IL-1 beta and tumor 
      necrosis factor-alpha increased secretion of all mediators. Release of IL-6 and GSCF 
      remained higher in nasal AEC compared with bronchial AEC following stimulation. 
      CONCLUSIONS: In epidemiological studies, nasal AEC may be a useful surrogate for 
      bronchial AEC for the study of RANTES, MCP-1, TIMP-1, and MMP-9 release in 
      children but bronchial AEC will remain the gold standard.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Pringle, Emily J
AU  - Pringle EJ
AD  - Child Health, University of Aberdeen, Aberdeen, UK.
FAU - Richardson, Helen B
AU  - Richardson HB
FAU - Miller, David
AU  - Miller D
FAU - Cornish, Daniella S
AU  - Cornish DS
FAU - Devereux, Graham S
AU  - Devereux GS
FAU - Walsh, Garry M
AU  - Walsh GM
FAU - Turner, Steve W
AU  - Turner SW
LA  - eng
PT  - Journal Article
DEP - 20120928
PL  - United States
TA  - Pediatr Pulmonol
JT  - Pediatric pulmonology
JID - 8510590
RN  - 0 (Cytokines)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adolescent
MH  - *Bronchi/cytology/metabolism
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/metabolism
MH  - Epithelial Cells/*metabolism
MH  - Female
MH  - Granulocyte Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Infant
MH  - Male
MH  - Matrix Metalloproteinase 9/metabolism
MH  - *Nasal Mucosa/cytology/metabolism
MH  - *Respiratory Mucosa/cytology/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2012/10/02 06:00
MHDA- 2013/05/22 06:00
CRDT- 2012/10/02 06:00
PHST- 2012/03/05 00:00 [received]
PHST- 2012/06/27 00:00 [accepted]
PHST- 2012/10/02 06:00 [entrez]
PHST- 2012/10/02 06:00 [pubmed]
PHST- 2013/05/22 06:00 [medline]
AID - 10.1002/ppul.22672 [doi]
PST - ppublish
SO  - Pediatr Pulmonol. 2012 Dec;47(12):1215-25. doi: 10.1002/ppul.22672. Epub 2012 Sep 
      28.