PMID- 23027005
OWN - NLM
STAT- MEDLINE
DCOM- 20130211
LR  - 20211021
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 931
DP  - 2013
TI  - Laser scanning cytometry: principles and applications-an update.
PG  - 187-212
LID - 10.1007/978-1-62703-056-4_11 [doi]
AB  - Laser scanning cytometer (LSC) is the microscope-based cytofluorometer that 
      offers a plethora of unique analytical capabilities, not provided by flow 
      cytometry (FCM). This review describes attributes of LSC and covers its numerous 
      applications derived from plentitude of the parameters that can be measured. 
      Among many LSC applications the following are emphasized: (a) assessment of 
      chromatin condensation to identify mitotic, apoptotic cells, or senescent cells; 
      (b) detection of nuclear or mitochondrial translocation of critical factors such 
      as NF-kappaB, p53, or Bax; (c) semi-automatic scoring of micronuclei in mutagenicity 
      assays; (d) analysis of fluorescence in situ hybridization (FISH) and use of the 
      FISH analysis attribute to measure other punctuate fluorescence patterns such as 
      gammaH2AX foci or receptor clustering; (e) enumeration and morphometry of nucleoli 
      and other cell organelles; (f) analysis of progeny of individual cells in 
      clonogenicity assay; (g) cell immunophenotyping; (h) imaging, visual examination, 
      or sequential analysis using different probes of the same cells upon their 
      relocation; (i) in situ enzyme kinetics, drug uptake, and other time-resolved 
      processes; (j) analysis of tissue section architecture using fluorescent and 
      chromogenic probes; (k) application for hypocellular samples (needle aspirate, 
      spinal fluid, etc.); and (l) other clinical applications. Advantages and 
      limitations of LSC are discussed and compared with FCM.
FAU - Pozarowski, Piotr
AU  - Pozarowski P
AD  - The Brander Cancer Research Institute, New York Medical College, Valhalla, NY, 
      USA.
FAU - Holden, Elena
AU  - Holden E
FAU - Darzynkiewicz, Zbigniew
AU  - Darzynkiewicz Z
LA  - eng
GR  - R01 CA028704/CA/NCI NIH HHS/United States
GR  - CA 28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - DNA Damage
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Laser Scanning Cytometry
MH  - Micronucleus Tests
MH  - Protein Transport
PMC - PMC3488462
MID - NIHMS414958
EDAT- 2012/10/03 06:00
MHDA- 2013/02/12 06:00
PMCR- 2014/01/01
CRDT- 2012/10/03 06:00
PHST- 2012/10/03 06:00 [entrez]
PHST- 2012/10/03 06:00 [pubmed]
PHST- 2013/02/12 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.1007/978-1-62703-056-4_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;931:187-212. doi: 10.1007/978-1-62703-056-4_11.