PMID- 23030636
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20131121
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 48
IP  - 3
DP  - 2013 Jun
TI  - Characterization and analysis of migration patterns of dentospheres derived from 
      periodontal tissue and the palate.
PG  - 276-85
LID - 10.1111/jre.12005 [doi]
AB  - BACKGROUND AND OBJECTIVE: Stem cells derived from periodontal and palatal tissues 
      may be useful for regenerative therapies of periodontal tissues. In addition to 
      the use of single periodontium-derived stem cells (pdSCs) and palatal-derived 
      stem cells (paldSCs), the application of pdSC and paldSC dentospheres, providing 
      a pool of vital stem cells, may be a useful approach. As cell migration is a 
      prerequisite for stem cells to regenerate a three-dimensional tissue environment, 
      we characterized pdSCs and paldSCs and investigated the migratory activity of 
      dentospheres within a three-dimensional environment. We also investigated the 
      capacity of the dentospheres to grow on zirconium dioxide surfaces. MATERIAL AND 
      METHODS: The capacity of pdSCs and paldSCs to differentiate into the neuronal and 
      osteogenic lineages was proved by RT-PCR and immunohistochemistry through the 
      detection of specific lineage markers, such as alkaline phosphatase, glutamate 
      decarboxylase 1 (also known as GAD67, the 67-kDa isoform of glutamate 
      decarboxylase), neurofilament-M and beta-III-tubulin. The expression profile of 
      surface molecules on pdSCs and paldSCs was analyzed by flow cytometry. Adhesion 
      and growth of pdSC/paldSC dentospheres on zirconium dioxide surfaces were 
      determined using confocal laser-scanning microscopy. The migratory behavior of 
      the cells was analyzed using a three-dimensional collagen matrix migration assay. 
      RESULTS: Both pdSCs and paldSCs were positive for epidermal growth factor 
      receptor, CC chemokine receptor 2 and CXC chemokine receptor 4 expression and 
      were able to grow on zirconium dioxide surfaces. Cell-migration experiments 
      revealed that both stem-cell populations responded similarly to epidermal growth 
      factor (EGF), monocyte chemotactic protein 1 (MCP-1) and stromal cell-derived 
      factor 1alpha (SDF-1alpha). Stimulation with EGF resulted in an increased migratory 
      activity of both stem-cell types, whereas the locomotory behavior of the cells 
      was impaired by both MCP-1 and SDF-1alpha. CONCLUSION: Dentospheres represent a pool 
      of vital pdSCs/paldSCs. As a result of the migratory activity demonstrated, along 
      with the capacity to grow on zirconium dioxide surfaces, dentospheres may be 
      useful for regenerative purposes in periodontal tissues.
CI  - (c) 2012 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Keeve, P L
AU  - Keeve PL
AD  - Department of Periodontology, School of Dentistry, ZBAF, Faculty of Health, 
      Witten/Herdecke University, Witten, Germany.
FAU - Dittmar, T
AU  - Dittmar T
FAU - Gassmann, G
AU  - Gassmann G
FAU - Grimm, W-D
AU  - Grimm WD
FAU - Niggemann, B
AU  - Niggemann B
FAU - Friedmann, A
AU  - Friedmann A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121003
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL12)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - C6V6S92N3C (Zirconium)
RN  - S38N85C5G0 (zirconium oxide)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - *Cell Movement/drug effects
MH  - Cell Proliferation
MH  - Chemokine CCL2/pharmacology
MH  - Chemokine CXCL12/pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Neurogenesis
MH  - Osteogenesis
MH  - Palate, Hard/*cytology
MH  - Periodontium/*cytology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/*cytology/drug effects/*physiology
MH  - Zirconium
EDAT- 2012/10/04 06:00
MHDA- 2013/10/01 06:00
CRDT- 2012/10/04 06:00
PHST- 2012/08/08 00:00 [accepted]
PHST- 2012/10/04 06:00 [entrez]
PHST- 2012/10/04 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
AID - 10.1111/jre.12005 [doi]
PST - ppublish
SO  - J Periodontal Res. 2013 Jun;48(3):276-85. doi: 10.1111/jre.12005. Epub 2012 Oct 
      3.