PMID- 23031343
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20200103
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 162
IP  - 2-4
DP  - 2013 Mar 23
TI  - Exploratory study of Torque teno sus viruses in pulmonary inflammatory lesions in 
      pigs.
PG  - 338-344
LID - S0378-1135(12)00509-3 [pii]
LID - 10.1016/j.vetmic.2012.09.011 [doi]
AB  - The pathogenic role of Torque teno sus viruses 1 (TTSuV1) and 2 (TTSuV2), and 
      their capacity to induce lesions are controversial. TTSuVs have been linked to 
      porcine circovirus diseases (PCVDs) and described as cause of mild respiratory 
      lesions in gnotobiotic pigs; moreover, an increased TTSuV prevalence has been 
      found in porcine respiratory disease complex (PRDC) affected pigs. In the present 
      study, TTSuV1 and TTSuV2 loads and their prevalence were evaluated in 
      formalin-fixed, paraffin-embedded (FFPE) lung samples displaying different types 
      of inflammatory lesions. Such measurements were made by means of a real time 
      quantitative PCR technique to detect these viruses. The technique was optimised 
      for its use on FFPE tissues comparing results with frozen lung tissues. Selection 
      criteria included negativity against porcine circovirus type 2 (PCV2), porcine 
      reproductive and respiratory syndrome virus (PRRSV), Aujeszky's disease virus 
      (ADV) and swine influenza virus (SIV). Results from the present study 
      demonstrated that both TTSuVs were present in lung. However, TTSuV2 had higher 
      viral load and prevalence in all the studied groups when compared to TTSuV1. 
      TTSuV2 mean load was also higher in lungs with viral background (interstitial 
      pneumonia and broncho-interstitial pneumonia) when compared to normal lungs or to 
      those with bacterial background (catarrhal-purulent bronchopneumonia, fibrinous 
      pleuritis and fibrinous-necrotizing pleuropneumonia). This result suggests a 
      possible role of TTSuV2 in the pathogenic mechanism of inflammatory lesions of 
      lungs compatible with viral infection.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Aramouni, M
AU  - Aramouni M
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: 
      mario.aramouni@cresa.uab.cat.
FAU - Martinez, J
AU  - Martinez J
AD  - Departament de Sanitat i Anatomia Animals, Facultat Veterinaria, Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
FAU - Nieto, D
AU  - Nieto D
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
FAU - Kekarainen, T
AU  - Kekarainen T
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
FAU - Segales, J
AU  - Segales J
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain; Departament de Sanitat 
      i Anatomia Animals, Facultat Veterinaria, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Barcelona, Spain. Electronic address: 
      joaquim.segales@cresa.uab.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120914
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - DNA Virus Infections/epidemiology/*veterinary/virology
MH  - Lung/virology
MH  - Paraffin Embedding
MH  - Pneumonia, Viral/epidemiology/*veterinary/virology
MH  - Prevalence
MH  - Real-Time Polymerase Chain Reaction
MH  - Spain/epidemiology
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/epidemiology/*virology
MH  - Torque teno virus/genetics/*isolation & purification
MH  - Viral Load
EDAT- 2012/10/04 06:00
MHDA- 2014/06/03 06:00
CRDT- 2012/10/04 06:00
PHST- 2012/06/19 00:00 [received]
PHST- 2012/09/06 00:00 [revised]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/10/04 06:00 [entrez]
PHST- 2012/10/04 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
AID - S0378-1135(12)00509-3 [pii]
AID - 10.1016/j.vetmic.2012.09.011 [doi]
PST - ppublish
SO  - Vet Microbiol. 2013 Mar 23;162(2-4):338-344. doi: 10.1016/j.vetmic.2012.09.011. 
      Epub 2012 Sep 14.