PMID- 23035093
OWN - NLM
STAT- MEDLINE
DCOM- 20130117
LR  - 20230425
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 32
IP  - 40
DP  - 2012 Oct 3
TI  - Optimization of a GCaMP calcium indicator for neural activity imaging.
PG  - 13819-40
LID - 10.1523/JNEUROSCI.2601-12.2012 [doi]
AB  - Genetically encoded calcium indicators (GECIs) are powerful tools for systems 
      neuroscience. Recent efforts in protein engineering have significantly increased 
      the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, 
      has been deployed in a number of model organisms and can reliably detect three or 
      more action potentials in short bursts in several systems in vivo. Through 
      protein structure determination, targeted mutagenesis, high-throughput screening, 
      and a battery of in vitro assays, we have increased the dynamic range of GCaMP3 
      by severalfold, creating a family of "GCaMP5" sensors. We tested GCaMP5s in 
      several systems: cultured neurons and astrocytes, mouse retina, and in vivo in 
      Caenorhabditis chemosensory neurons, Drosophila larval neuromuscular junction and 
      adult antennal lobe, zebrafish retina and tectum, and mouse visual cortex. 
      Signal-to-noise ratio was improved by at least 2- to 3-fold. In the visual 
      cortex, two GCaMP5 variants detected twice as many visual stimulus-responsive 
      cells as GCaMP3. By combining in vivo imaging with electrophysiology we show that 
      GCaMP5 fluorescence provides a more reliable measure of neuronal activity than 
      its predecessor GCaMP3. GCaMP5 allows more sensitive detection of neural activity 
      in vivo and may find widespread applications for cellular imaging in general.
FAU - Akerboom, Jasper
AU  - Akerboom J
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 
      20147, USA.
FAU - Chen, Tsai-Wen
AU  - Chen TW
FAU - Wardill, Trevor J
AU  - Wardill TJ
FAU - Tian, Lin
AU  - Tian L
FAU - Marvin, Jonathan S
AU  - Marvin JS
FAU - Mutlu, Sevinc
AU  - Mutlu S
FAU - Calderon, Nicole Carreras
AU  - Calderon NC
FAU - Esposti, Federico
AU  - Esposti F
FAU - Borghuis, Bart G
AU  - Borghuis BG
FAU - Sun, Xiaonan Richard
AU  - Sun XR
FAU - Gordus, Andrew
AU  - Gordus A
FAU - Orger, Michael B
AU  - Orger MB
FAU - Portugues, Ruben
AU  - Portugues R
FAU - Engert, Florian
AU  - Engert F
FAU - Macklin, John J
AU  - Macklin JJ
FAU - Filosa, Alessandro
AU  - Filosa A
FAU - Aggarwal, Aman
AU  - Aggarwal A
FAU - Kerr, Rex A
AU  - Kerr RA
FAU - Takagi, Ryousuke
AU  - Takagi R
FAU - Kracun, Sebastian
AU  - Kracun S
FAU - Shigetomi, Eiji
AU  - Shigetomi E
FAU - Khakh, Baljit S
AU  - Khakh BS
FAU - Baier, Herwig
AU  - Baier H
FAU - Lagnado, Leon
AU  - Lagnado L
FAU - Wang, Samuel S-H
AU  - Wang SS
FAU - Bargmann, Cornelia I
AU  - Bargmann CI
FAU - Kimmel, Bruce E
AU  - Kimmel BE
FAU - Jayaraman, Vivek
AU  - Jayaraman V
FAU - Svoboda, Karel
AU  - Svoboda K
FAU - Kim, Douglas S
AU  - Kim DS
FAU - Schreiter, Eric R
AU  - Schreiter ER
FAU - Looger, Loren L
AU  - Looger LL
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - MC_U105178794/MRC_/Medical Research Council/United Kingdom
GR  - R01 NS045193/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/chemistry/ultrastructure
MH  - Caenorhabditis elegans
MH  - *Calcium Signaling
MH  - Crystallography, X-Ray
MH  - Drosophila melanogaster/growth & development
MH  - Female
MH  - Fluorescent Dyes/analysis/*chemistry
MH  - Fluorometry/*methods
MH  - Genes, Synthetic
MH  - Genetic Vectors
MH  - Green Fluorescent Proteins/analysis/*chemistry/genetics/isolation & purification
MH  - HEK293 Cells/chemistry/ultrastructure
MH  - Hippocampus/chemistry/cytology
MH  - Humans
MH  - Larva
MH  - Lasers
MH  - Mice
MH  - Models, Molecular
MH  - Mutagenesis, Site-Directed
MH  - Neuroimaging/*methods
MH  - Neuromuscular Junction/chemistry/ultrastructure
MH  - Neurons/*chemistry/physiology/ultrastructure
MH  - Neuropil/chemistry/physiology/ultrastructure
MH  - Olfactory Receptor Neurons/chemistry/physiology/ultrastructure
MH  - Peptides/analysis/*chemistry/genetics
MH  - Photic Stimulation
MH  - Protein Conformation
MH  - Rats
MH  - Recombinant Fusion Proteins/analysis/chemistry/genetics
MH  - Retinal Bipolar Cells/chemistry/physiology/ultrastructure
MH  - *Synaptic Transmission
MH  - Zebrafish/growth & development
PMC - PMC3482105
MID - NIHMS412860
OID - NLM: HHMIMS412860
EDAT- 2012/10/05 06:00
MHDA- 2013/01/18 06:00
PMCR- 2013/04/03
CRDT- 2012/10/05 06:00
PHST- 2012/10/05 06:00 [entrez]
PHST- 2012/10/05 06:00 [pubmed]
PHST- 2013/01/18 06:00 [medline]
PHST- 2013/04/03 00:00 [pmc-release]
AID - 32/40/13819 [pii]
AID - 3801135 [pii]
AID - 10.1523/JNEUROSCI.2601-12.2012 [doi]
PST - ppublish
SO  - J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 10.1523/JNEUROSCI.2601-12.2012.