PMID- 23036593 OWN - NLM STAT- MEDLINE DCOM- 20130211 LR - 20121018 IS - 1469-8161 (Electronic) IS - 0031-1820 (Linking) VI - 139 IP - 12 DP - 2012 Oct TI - A previously undescribed ostreid herpes virus 1 (OsHV-1) genotype detected in the pacific oyster, Crassostrea gigas, in Ireland. PG - 1526-32 LID - 10.1017/S0031182012000881 [doi] AB - Significant mortalities of the Pacific oyster, Crassostrea gigas, have been reported worldwide since the 1950s. The impact these re-occurring mortality events have had on the C. gigas industry has highlighted the necessity to determine the factors that may be causing these mortalities. This study investigated the possible role of ostreid herpes virus (OsHV-1) in C. gigas mortalities over 2 successive summers at 2 study areas in Ireland. A single sample of adult C. gigas, which had been experiencing mortalities at one of the sites was screened. Successive cohorts of C. gigas spat obtained from a hatchery outside Ireland was relayed to both sites in 2003 and in 2004. Spat were screened each year prior to relaying. Samples were collected every 2 weeks and mortality counts were recorded and observed at both sites. Polymerase chain reaction (PCR) analysis and subsequent sequencing indicated that a previously undocumented variant genotype of OsHV-1 was present in the single cohort of adult C. gigas and in seed and juveniles at both sites, in both years. Analysis suggests that the Irish OsHV-1 muvar variant genotype is closely related to OsHV-1 muvar, first described in France in 2008. FAU - Lynch, S A AU - Lynch SA AD - Aquaculture & Fisheries Development Centre, School of Biological, Earth & Environmental Sciences, University College Cork, The Cooperage, Distillery Fields, North Mall, Cork, Ireland. s.lynch@ucc.ie FAU - Carlsson, J AU - Carlsson J FAU - Reilly, A O AU - Reilly AO FAU - Cotter, E AU - Cotter E FAU - Culloty, S C AU - Culloty SC LA - eng SI - GENBANK/JQ963169 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120719 PL - England TA - Parasitology JT - Parasitology JID - 0401121 RN - 0 (DNA, Viral) SB - IM MH - Animals MH - Base Sequence MH - Crassostrea/virology MH - DNA, Viral/genetics MH - Genotype MH - Herpesviridae/*classification/*genetics MH - Ireland MH - Molecular Sequence Data MH - Phylogeny MH - Polymerase Chain Reaction MH - Risk Factors MH - Sequence Alignment MH - Species Specificity MH - Survival Analysis EDAT- 2012/10/06 06:00 MHDA- 2013/02/12 06:00 CRDT- 2012/10/06 06:00 PHST- 2012/10/06 06:00 [entrez] PHST- 2012/10/06 06:00 [pubmed] PHST- 2013/02/12 06:00 [medline] AID - S0031182012000881 [pii] AID - 10.1017/S0031182012000881 [doi] PST - ppublish SO - Parasitology. 2012 Oct;139(12):1526-32. doi: 10.1017/S0031182012000881. Epub 2012 Jul 19.