PMID- 23037972
OWN - NLM
STAT- MEDLINE
DCOM- 20130204
LR  - 20121210
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 139
IP  - 1
DP  - 2013
TI  - Characterization and genome organization of a repetitive element associated with 
      the nucleolus organizer region in Leporinus elongatus (Anostomidae: 
      Characiformes).
PG  - 22-8
LID - 10.1159/000342957 [doi]
AB  - Chromosome mapping and studies of the genomic organization of repetitive DNA 
      sequences provide valuable insights that enhance our evolutionary and structural 
      understanding of these sequences, as well as identifying chromosomal 
      rearrangements and sex determination. This study investigated the occurrence and 
      organization of repetitive DNA sequences in Leporinus elongatus using restriction 
      enzyme digestion and the mapping of sequences by chromosomal fluorescence in situ 
      hybridization (FISH). A 378-bp fragment with a 54.2% GC content was isolated 
      after digestion with the SmaI restriction enzyme. BLASTN search found no 
      similarity with previously described sequences, so this repetitive sequence was 
      named LeSmaI. FISH experiments were conducted using L. elongatus and other 
      Anostomidae species, i.e. L. macrocephalus, L. obtusidens, L. striatus, L. 
      lacustris, L. friderici, Schizodon borellii, S. isognathus, and Abramites 
      hypselonotus which detected signals that were unique to male and female L. 
      elongatus individuals. Double-FISH using LeSmaI and 18S rDNA showed that LeSmaI 
      was located in a nucleolus organizer region (NOR) in the male and female 
      metaphases of L. elongatus. This report also discusses the role of repetitive DNA 
      associated with NORs in the diversification of Anostomidae species karyotypes.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - da Silva, E L
AU  - da Silva EL
AD  - Laboratorio de Citogenetica, Departamento de Biologia, Instituto de Biociencias, 
      UNESP-Universidade Estadual Paulista Julio de Mesquita Filho, Rio Claro, Brasil.
FAU - Busso, A F
AU  - Busso AF
FAU - Parise-Maltempi, P P
AU  - Parise-Maltempi PP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121002
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Characiformes/*genetics
MH  - Chromosome Mapping
MH  - Female
MH  - *Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Nucleolus Organizer Region/*genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 2012/10/06 06:00
MHDA- 2013/02/05 06:00
CRDT- 2012/10/06 06:00
PHST- 2012/07/07 00:00 [accepted]
PHST- 2012/10/06 06:00 [entrez]
PHST- 2012/10/06 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
AID - 000342957 [pii]
AID - 10.1159/000342957 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2013;139(1):22-8. doi: 10.1159/000342957. Epub 2012 Oct 2.