PMID- 23039253
OWN - NLM
STAT- MEDLINE
DCOM- 20130125
LR  - 20220316
IS  - 1742-4658 (Electronic)
IS  - 1742-464X (Linking)
VI  - 279
IP  - 23
DP  - 2012 Dec
TI  - DNA demethylation regulates the expression of miR-210 in neural progenitor cells 
      subjected to hypoxia.
PG  - 4318-26
LID - 10.1111/febs.12021 [doi]
AB  - Several studies have identified a set of hypoxia-regulated microRNAs, among which 
      is miR-210, whose expression is highly induced by hypoxia in various cancer cell 
      lines. Recent studies have highlighted the importance of miR-210 and its 
      transcriptional regulation by the transcription factor hypoxia-inducible factor-1 
      (HIF-1). We report here that the expression of miR-210 was highly induced in 
      neural progenitor cells (NPCs) subjected to hypoxia. Specifically, treating 
      hypoxic NPCs with the DNA demethylating agent 5-aza-2'-deoxycytidine 
      significantly increased the expression of miR-210, even under normoxia; however, 
      the activity of hypoxia-inducible factor-1 was unaffected. Further analysis of 
      the miR-210 sequence revealed that it is embedded in a CpG island. Bisulfite 
      sequencing of the miR-210 CpG island from NPCs grown under hypoxic conditions 
      showed 24% CpG methylation in NPCs exposed to 20% O(2) , 18% in NPCs exposed to 
      3% O(2) , and 12% in NPCs exposed to 0.3% O(2) . In addition, the activity of DNA 
      methyltransferases (DNMTs) in NPCs decreased after exposure to hypoxia. 
      Specifically, the expression of DNMT3b decreased significantly after exposure to 
      0.3% O(2) . Thus, these results demonstrate that DNA demethylation regulates 
      miR-210 expression in NPCs under both normoxia and hypoxia.
CI  - (c) 2012 The Authors Journal compilation (c) 2012 FEBS.
FAU - Xiong, Lei
AU  - Xiong L
AD  - Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing, 
      China; College of Life Science, Wuhan University, Wuhan, China.
FAU - Wang, Fei
AU  - Wang F
FAU - Huang, Xin
AU  - Huang X
FAU - Liu, Zhao-hui
AU  - Liu ZH
FAU - Zhao, Tong
AU  - Zhao T
FAU - Wu, Li-ying
AU  - Wu LY
FAU - Wu, Kuiwu
AU  - Wu K
FAU - Ding, Xuefeng
AU  - Ding X
FAU - Liu, Shuhong
AU  - Liu S
FAU - Wu, Yan
AU  - Wu Y
FAU - Zhao, Yongqi
AU  - Zhao Y
FAU - Zhu, Ling-ling
AU  - Zhu LL
FAU - Fan, Ming
AU  - Fan M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121107
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (MIRN210 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 776B62CQ27 (Decitabine)
RN  - EC 2.1.1.- (DNA Modification Methylases)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Animals
MH  - Azacitidine/analogs & derivatives/pharmacology
MH  - Blotting, Western
MH  - Cell Hypoxia/genetics/*physiology
MH  - Cells, Cultured
MH  - DNA Methylation/drug effects/genetics/*physiology
MH  - DNA Modification Methylases/genetics/metabolism
MH  - Decitabine
MH  - Female
MH  - Hypoxia-Inducible Factor 1/genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Neural Stem Cells/*metabolism
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
EDAT- 2012/10/09 06:00
MHDA- 2013/01/26 06:00
CRDT- 2012/10/09 06:00
PHST- 2012/06/29 00:00 [received]
PHST- 2012/08/15 00:00 [revised]
PHST- 2012/10/03 00:00 [accepted]
PHST- 2012/10/09 06:00 [entrez]
PHST- 2012/10/09 06:00 [pubmed]
PHST- 2013/01/26 06:00 [medline]
AID - 10.1111/febs.12021 [doi]
PST - ppublish
SO  - FEBS J. 2012 Dec;279(23):4318-26. doi: 10.1111/febs.12021. Epub 2012 Nov 7.