PMID- 23040778
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20161125
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 24
IP  - 10
DP  - 2012 Oct
TI  - [Effects of unfractionated heparin on expression of metalloproteinase-2 and -9 in 
      acute lung injury rats].
PG  - 608-11
AB  - OBJECTIVE: To determine the activation of matrix metalloproteinase (MMP-2, MMP-9) 
      in rats with acute lung injury (ALI) and the effects of unfractionated heparin 
      (UFH) on the levels. METHODS: Eighteen male Wistar rats were divided into control 
      group, ALI group and UFH group, with 6 rats in each group by means of random 
      number table. ALI was induced by administering a bolus injection of 
      lipopolysaccharide (LPS) via the caudal vein at a dose of 6 mg/kg. In UFH group 
      the rats were treated intravenously with 100 U/kg of UFH 15 minutes before the 
      injection of LPS. In control group, the rats were treated with the same volume of 
      normal saline. Serum levels of MMP-2 and MMP-9 were measured by enzyme linked 
      immunosorbent assay (ELISA) at 1, 3, 6 hours via femoral vein. Six hours after 
      the injection of reagents, the rats were sacrificed and lung tissue samples were 
      collected for mRNA analysis of the MMP-2 and MMP-9 by real-time fluorescence 
      quantitate reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS: 
      Compared with control group, the content of MMP-2 and MMP-9 in serum of ALI group 
      were increased, and reached the peak at 6 hours (MMP-2: 2.86+/-0.40 mug/L vs. 
      1.21+/-0.24 mug/L, MMP-9: 2.54+/-0.29 mug/L vs. 1.15+/-0.34 mug/L, both P<0.01); they were 
      down-regulated in UFH group at 6 hours (MMP-2: 1.92+/-0.31 mug/L vs. 2.86+/-0.40 mug/L, 
      MMP-9: 1.82+/-0.26 mug/L vs. 2.54+/-0.29 mug/L, both P<0.05). Compared with control 
      group, the mRNA expressions of MMP-2 and MMP-9 in the lung tissue of ALI group 
      were increased at 6 hours (MMP-2 mRNA: 1.88+/-0.09 vs. 1.00+/-0.10, MMP-9 mRNA: 
      3.15+/-0.47 vs. 1.00+/-0.17, both P<0.01); they were down-regulated in UFH group 
      (MMP-2 mRNA: 1.26+/-0.14 vs. 1.88+/-0.09, P<0.01; MMP-9 mRNA: 2.06+/-0.68 vs. 
      3.15+/-0.47, P<0.05), but still above the control group (both P<0.05). CONCLUSIONS: 
      The present study demonstrated that the level of MMP-2 and MMP-9 increased in 
      rats with ALI. UFH could exert protective effects by inhibiting expression of 
      MMP-2 and MMP-9 in serum and lung tissue, in both mRNA and protein expression.
FAU - Li, Xu
AU  - Li X
AD  - Department of Intensive Care Unit, China Medical University, Liaoning, China.
FAU - Zheng, Zhen
AU  - Zheng Z
FAU - Ma, Xiao-chun
AU  - Ma XC
LA  - chi
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.24 (Mmp2 protein, rat)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, rat)
SB  - IM
MH  - Acute Lung Injury/*metabolism
MH  - Animals
MH  - Heparin/*pharmacology
MH  - Male
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2012/10/09 06:00
MHDA- 2013/08/02 06:00
CRDT- 2012/10/09 06:00
PHST- 2012/10/09 06:00 [entrez]
PHST- 2012/10/09 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2012 Oct;24(10):608-11.