PMID- 23040779
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20121008
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 24
IP  - 10
DP  - 2012 Oct
TI  - [The effects of three dosages of nebulized unfractionated heparin on alveolar 
      coagulation and tissue inflammation injury in endotoxin-induced acute lung injury 
      rat model].
PG  - 612-5
AB  - OBJECTIVE: To observe the effects of three dosages of nebulized unfractionated 
      heparin (UFH) on alveolar coagulation, inflammation and lung histology in 
      endotoxin-induced acute lung injury rat model, and investigate the appropriated 
      dose of local UFH in managing intrapulmonary coagulopathy. METHODS: Twenty-nine 
      male Wistar rats were divided into control (n=5) and UFH group (n=24) in table of 
      random number, which were duplicated to be endotoxin-induced ALI rat model with 
      lipopolysaccharide (LPS) injecting by intravenous route. The UFH group was 
      divided into three subgroups, which were administered once with 6, 12 and 18 U/g 
      aerosolized UFH in 10 ml at 2 hours after challenge, respectively, while the 
      control group was simply nebulized with normal saline. All rats were sacrificed 
      at 6 hours after intravenous administration of LPS, bronchoalveolar lavage was 
      performed, and the fluid was collected. Enzyme-linked immune sorbent assay 
      (ELISA) was used to measure the level of thrombin-antithrombin complex (TATc), 
      tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid (BALF), and lung 
      wet/dry (W/D) weight ratio, histology score were recorded. RESULTS: At 6 hours 
      after LPS-induced lung injury, the levels of TATc and TNF-alpha, lung W/D weight 
      ratio and histology score in 6 U/g and 12 U/g group were all lower than those of 
      control group significantly (TATc: 0.959+/-0.681 mug/L, 1.165+/-0.854 mug/L vs. 
      2.141+/-0.791 mug/L, TNF-alpha: 4.449+/-5.054 ng/L, 9.096+/-4.099 ng/L vs. 18.184+/-3.869 
      ng/L, W/D weight ratio: 7.018+/-1.137, 7.367+/-0.349 vs. 8.472+/-0.614, histology 
      score: 16.0+/-1.0, 16.5+/-1.5 vs. 19.6+/-0.4, P<0.05 or P<0.01). There was no 
      significant difference in the comparisons between the subgroups of UFH in TATc 
      level in BALF and lung histology score. For the TNF-alphalevel in BALF, 18 U/g group 
      evidently exceeded that of 6 U/g group (15.503+/-8.753 ng/L vs. 4.449+/-5.054 ng/L, 
      P<0.01), and lung W/D weight ratio in 18 U/g group was also significantly higher 
      comparing to 6 U/g (8.850+/-1.157 vs. 7.018+/-1.137, P<0.05) and 12 U/g group 
      (8.850+/-1.157 vs. 7.367+/-0.349, P<0.05). CONCLUSION: It was appropriate for the 
      dose of nebulized UFH to be administered no more than 12 U/g in ALI treatment, 
      which was enough to inhibit alveolar coagulant cascade, decrease early 
      inflammatory response and alleviate lung tissue injury.
FAU - Wang, Zong-yu
AU  - Wang ZY
AD  - Department of Critical Care Medicine, Peking University, Beijing, China.
FAU - Wu, Sheng-nan
AU  - Wu SN
FAU - Zhu, Xi
AU  - Zhu X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Endotoxins)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Acute Lung Injury/chemically induced/*drug therapy/*pathology
MH  - Administration, Inhalation
MH  - Animals
MH  - Blood Coagulation/drug effects
MH  - Endotoxins/adverse effects
MH  - Heparin/*administration & dosage/pharmacology
MH  - Inflammation/drug therapy
MH  - Lung/physiopathology
MH  - Male
MH  - Pulmonary Alveoli/drug effects
MH  - Rats
MH  - Rats, Wistar
EDAT- 2012/10/09 06:00
MHDA- 2013/08/02 06:00
CRDT- 2012/10/09 06:00
PHST- 2012/10/09 06:00 [entrez]
PHST- 2012/10/09 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2012 Oct;24(10):612-5.