PMID- 23040781
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20131121
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 24
IP  - 10
DP  - 2012 Oct
TI  - [The effects of postconditioning with propofol on Toll-like receptor 4 expression 
      in the lung tissue of rat with acute lung injury].
PG  - 620-3
AB  - OBJECTIVE: To investigate the effect of postconditioning with propofol on 
      Toll-like receptor 4 (TLR4) expression in the lung tissue in lipopolysaccharide 
      (LPS)-induced acute lung injury (ALI) rats. METHODS: Thirty Sprague-Dawley (SD) 
      rats were randomly assigned to control group, ALI group, and propofol 
      postcondition group (each n=10). The model of ALI was reproduced by intravenous 
      injection of LPS (8 mg/kg for 30 minutes) into the rats, equivalent normal saline 
      was injected into the rats of control group. The rats were postconditioned with 
      propofol injected intravenously by 20 mg/kg bolus dose and then continuously by 
      40 mgxkg(-1)xh(-1) with a constant speed for 1 hour. The rats were sacrificed 6 
      hours after drug injection. Lung wet/dry weight (W/D) ratio and lung permeability 
      index (LPI) was taken. Tumor necrosis factor-alpha (TNF-alpha) level in bronchoalveolar 
      lavage fluid (BALF) was detected using enzyme linked immunosorbent assay (ELISA) 
      method and TLR4 mRNA expression in lung tissue was assessed by reverse 
      transcription-polymerase chain reaction (RT-PCR). RESULTS: The lung W/D ratio, 
      LPI, TLR4 mRNA and TNF-alpha in BALF were all increased in ALI group compared with 
      control group [lung W/D ratio: 5.30+/-0.28 vs. 4.21+/-0.14, LPI (x10(-3)): 8.7+/-2.2 
      vs. 3.3+/-2.0, TLR4 mRNA: 2.451+/-0.028 vs. 0.998+/-0.021, TNF-alpha: 643.46+/-62.31 ng/L vs. 
      120.43+/-12.65 ng/L, all P<0.05]. The above indexes were significantly reduced in 
      the propofol group than those in the ALI group [lung W/D ratio: 4.68+/-0.19 vs. 
      5.30+/-0.28, LPI (x10(-3)): 5.8+/-2.0 vs. 8.7+/-2.2, TLR4 mRNA: 1.126+/-0.025 vs. 
      2.451+/-0.028, TNF-alpha: 290.53+/-32.01 ng/L vs. 643.46+/-62.31 ng/L, all P<0.05], but 
      still higher than those in control group (all P<0.05). CONCLUSION: 
      Postconditioning with propofol may alleviate ALI via reducing TLR4 mRNA 
      expression, and inhibit the waterfall-like inflammatory reaction.
FAU - Li, Guo-Fu
AU  - Li GF
AD  - Department of Critical Care Medicine, China Medical University, Shenyang, 
      Liaoning, China. ligf@sj-hospital.org
FAU - Tong, Xin
AU  - Tong X
FAU - Luan, Ting
AU  - Luan T
FAU - Zang, Bin
AU  - Zang B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - YI7VU623SF (Propofol)
SB  - IM
MH  - Acute Lung Injury/*metabolism
MH  - Animals
MH  - Lung/drug effects/*metabolism
MH  - Male
MH  - Propofol/*pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 4/*metabolism
EDAT- 2012/10/09 06:00
MHDA- 2013/08/02 06:00
CRDT- 2012/10/09 06:00
PHST- 2012/10/09 06:00 [entrez]
PHST- 2012/10/09 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2012 Oct;24(10):620-3.