PMID- 2304144
OWN - NLM
STAT- MEDLINE
DCOM- 19900326
LR  - 20220228
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 64
IP  - 3
DP  - 1990 Mar
TI  - Sequence variants of human T-cell lymphotropic virus type I from patients with 
      tropical spastic paraparesis and adult T-cell leukemia do not distinguish 
      neurological from leukemic isolates.
PG  - 1278-82
AB  - We have amplified and sequenced DNA in the envelope (env) and long terminal 
      repeat (LTR) regions of human T-cell lymphotropic virus type I (HTLV-I) 
      proviruses from the peripheral blood of 10 HTLV-I-seropositive patients with 
      tropical spastic paraparesis (TSP) and two patients with adult T-cell leukemia. 
      The aim was to examine variation in these regions and to test the hypothesis that 
      the sequences of leukemogenic HTLV-I isolates differ from those causing the 
      neurological disease TSP. In 5 of the 12 HTLV-I-seropositive patients, more than 
      one HTLV-I sequence variant was identified in the same individual. No two 
      individuals shared identical sequences in either env or LTR U3. Sequence 
      variations were found at 73 positions in 1,416 bases amplified in env. Sequence 
      variability was found throughout the LTR-U3 region, including the sequences of 
      two transcriptional enhancers. Several nucleotide changes common to both 
      Caribbean and Japanese HTLV-I isolates allowed us to identify a consensus 
      sequence that differs from the HTLV-I prototype sequence (M. Seiki, S. Hattori, 
      Y. Hirayama, and M. Yoshida, Proc. Natl. Acad. Sci. USA 80:3618-3622, 1983). No 
      sequence in the env or LTR U3 region was found to be characteristic of isolates 
      from TSP patients. Although each isolate was distinct at the nucleotide level, 
      the predicted protein sequence of HTLV-I env is less variable than that of human 
      immunodeficiency virus env, suggesting that these lymphotropic retroviruses use 
      different strategies to evade host immune responses.
FAU - Daenke, S
AU  - Daenke S
AD  - Institute for Molecular Medicine, John Radcliffe Hospital, Oxford, United 
      Kingdom.
FAU - Nightingale, S
AU  - Nightingale S
FAU - Cruickshank, J K
AU  - Cruickshank JK
FAU - Bangham, C R
AU  - Bangham CR
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Female
MH  - Genes, Viral
MH  - *Genetic Variation
MH  - Human T-lymphotropic virus 1/*genetics/isolation & purification
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/*microbiology
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Paraparesis, Tropical Spastic/*microbiology
MH  - Polymerase Chain Reaction
MH  - Viral Envelope Proteins/genetics
PMC - PMC249244
EDAT- 1990/03/01 00:00
MHDA- 1990/03/01 00:01
PMCR- 1990/03/01
CRDT- 1990/03/01 00:00
PHST- 1990/03/01 00:00 [pubmed]
PHST- 1990/03/01 00:01 [medline]
PHST- 1990/03/01 00:00 [entrez]
PHST- 1990/03/01 00:00 [pmc-release]
AID - 10.1128/JVI.64.3.1278-1282.1990 [doi]
PST - ppublish
SO  - J Virol. 1990 Mar;64(3):1278-82. doi: 10.1128/JVI.64.3.1278-1282.1990.