PMID- 23041782
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20211021
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Print)
IS  - 1742-7061 (Linking)
VI  - 9
IP  - 1
DP  - 2013 Jan
TI  - A chondroitinase-ABC and TGF-beta1 treatment regimen for enhancing the mechanical 
      properties of tissue-engineered fibrocartilage.
PG  - 4626-34
LID - S1742-7061(12)00474-6 [pii]
LID - 10.1016/j.actbio.2012.09.037 [doi]
AB  - The development of functionally equivalent fibrocartilage remains elusive despite 
      efforts to engineer tissues such as knee meniscus, intervertebral disc and 
      temporomandibular joint disc. Attempts to engineer these structures often fail to 
      create tissues with mechanical properties on a par with native tissue, resulting 
      in constructs unsuitable for clinical applications. The objective of this study 
      was to engineer a spectrum of biomimetic fibrocartilages representative of the 
      distinct functional properties found in native tissues. Using the self-assembly 
      process, different co-cultures of meniscus cells and articular chondrocytes were 
      seeded into agarose wells and treated with the catabolic agent chondroitinase-ABC 
      (C-ABC) and the anabolic agent transforming growth factor-beta1 (TGF-beta1) via a 
      two-factor (cell ratio and bioactive treatment), full factorial study design. 
      Application of both C-ABC and TGF-beta1 resulted in a beneficial or positive 
      increase in the collagen content of treated constructs compared to controls. 
      Significant increases in both the collagen density and fiber diameter were also 
      seen with this treatment, increasing these values by 32 and 15%, respectively, 
      over control values. Mechanical testing found the combined bioactive treatment to 
      synergistically increase the Young's modulus and ultimate tensile strength of the 
      engineered fibrocartilages compared to controls, with values reaching the lower 
      spectrum of those found in native tissues. Together, these data demonstrate that 
      C-ABC and TGF-beta1 interact to develop a denser collagen matrix better able to 
      withstand tensile loading. This study highlights a way to optimize the tensile 
      properties of engineered fibrocartilage using a biochemical and a biophysical 
      agent together to create distinct fibrocartilages with functional properties 
      mimicking those of native tissue.
CI  - Copyright (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - MacBarb, Regina F
AU  - MacBarb RF
AD  - Department of Biomedical Engineering, University of California Davis, Davis, CA 
      95616, USA.
FAU - Makris, Eleftherios A
AU  - Makris EA
FAU - Hu, Jerry C
AU  - Hu JC
FAU - Athanasiou, Kyriacos A
AU  - Athanasiou KA
LA  - eng
GR  - R01 DE019666/DE/NIDCR NIH HHS/United States
GR  - T32 GM008799/GM/NIGMS NIH HHS/United States
GR  - R01 DEO19666/PHS HHS/United States
GR  - 5-T32-GM008799/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121004
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Cattle
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Coculture Techniques
MH  - *Fibrocartilage
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Scanning
MH  - *Tissue Engineering
MH  - Transforming Growth Factor beta1/*pharmacology
PMC - PMC3518537
MID - NIHMS412695
EDAT- 2012/10/09 06:00
MHDA- 2013/06/12 06:00
PMCR- 2014/01/01
CRDT- 2012/10/09 06:00
PHST- 2012/05/31 00:00 [received]
PHST- 2012/08/24 00:00 [revised]
PHST- 2012/09/27 00:00 [accepted]
PHST- 2012/10/09 06:00 [entrez]
PHST- 2012/10/09 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - S1742-7061(12)00474-6 [pii]
AID - 10.1016/j.actbio.2012.09.037 [doi]
PST - ppublish
SO  - Acta Biomater. 2013 Jan;9(1):4626-34. doi: 10.1016/j.actbio.2012.09.037. Epub 
      2012 Oct 4.