PMID- 23043979
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20121127
IS  - 1090-2449 (Electronic)
IS  - 0014-4894 (Linking)
VI  - 132
IP  - 4
DP  - 2012 Dec
TI  - Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster 
      liver slices infected with Entamoeba histolytica.
PG  - 424-33
LID - S0014-4894(12)00291-3 [pii]
LID - 10.1016/j.exppara.2012.09.012 [doi]
AB  - Precision-cut liver slices (PCLS) are mainly used to evaluate hepatotoxicity and 
      metabolism of chemicals, as well as to study mechanisms of liver damage and 
      repair. However, recently they have been used as a system to study amoebic 
      infections. The aim of this study was to validate this model as an alternative 
      for experimental amoebic liver absess (ALA) in animals. To do this, the PCLS was 
      analyzed for the expression of amoebapore and cysteine proteinases 1 and 5, three 
      of the most studied virulence factors of Entamoeba histolytica, as well as the 
      induction of apoptosis and cytokines production in response to the ex vivo 
      infection. PCHLS were prepared with the Brendel-Vitron tissue slicer and then, 
      infected with 200,000 trophozoites of E. histolytica. Samples were taken at 0, 6, 
      12, 18, and 24 h and compared to control non-infected slices. Morphological 
      studies were performed in order to verify the infection; while apoptosis was 
      studied by TUNEL and PAS techniques. The expression of cysteine proteinases (1 
      and 5), and amoebapore, was analyzed by real-time PCR. By using ELISA assays, the 
      production of cytokines was also studied. PCHLS were found to be a reproducible 
      infection system, and E. histolytica caused the expression of cysteine 
      proteinases and amoebapore in infected slices. At the same time, trophozoites 
      induce release of cytokines and apoptotic death of the hepatocytes close to them. 
      PCHLS represent a new and suitable alternative model to study the pathogenesis of 
      hepatic amoebiasis.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Carranza-Rosales, Pilar
AU  - Carranza-Rosales P
AD  - Centro de Investigacion Biomedica del Noreste, Instituto Mexicano del Seguro 
      Social, Administracion de Correos No. 4, Apartado Postal 020, Colonia 
      Independencia, Monterrey, NL, CP 64720, Mexico. pilarcarranza@cibinmty.net
FAU - Santiago-Mauricio, Maria Guadalupe
AU  - Santiago-Mauricio MG
FAU - Guzman-Delgado, Nancy Elena
AU  - Guzman-Delgado NE
FAU - Vargas-Villarreal, Javier
AU  - Vargas-Villarreal J
FAU - Lozano-Garza, Gerardo
AU  - Lozano-Garza G
FAU - Viveros-Valdez, Ezequiel
AU  - Viveros-Valdez E
FAU - Ortiz-Lopez, Rocio
AU  - Ortiz-Lopez R
FAU - Moran-Martinez, Javier
AU  - Moran-Martinez J
FAU - Gandolfi, A Jay
AU  - Gandolfi AJ
LA  - eng
PT  - Journal Article
DEP - 20121005
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Cytokines)
RN  - 0 (Virulence Factors)
RN  - EC 3.4.- (Cysteine Proteases)
SB  - IM
MH  - Animal Testing Alternatives/methods
MH  - Animals
MH  - *Apoptosis
MH  - Cricetinae
MH  - Cysteine Proteases/genetics/metabolism
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Entamoeba histolytica/immunology/*pathogenicity
MH  - Gene Expression Regulation, Enzymologic
MH  - In Situ Nick-End Labeling
MH  - Liver/immunology/*parasitology/pathology
MH  - Male
MH  - Mesocricetus
MH  - Periodic Acid-Schiff Reaction
MH  - Real-Time Polymerase Chain Reaction
MH  - Virulence Factors/analysis/genetics/*metabolism
EDAT- 2012/10/10 06:00
MHDA- 2013/01/25 06:00
CRDT- 2012/10/10 06:00
PHST- 2012/04/25 00:00 [received]
PHST- 2012/09/20 00:00 [accepted]
PHST- 2012/10/10 06:00 [entrez]
PHST- 2012/10/10 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - S0014-4894(12)00291-3 [pii]
AID - 10.1016/j.exppara.2012.09.012 [doi]
PST - ppublish
SO  - Exp Parasitol. 2012 Dec;132(4):424-33. doi: 10.1016/j.exppara.2012.09.012. Epub 
      2012 Oct 5.