PMID- 23045102
OWN - NLM
STAT- MEDLINE
DCOM- 20130225
LR  - 20161021
IS  - 1934-9262 (Electronic)
IS  - 1934-9254 (Linking)
VI  - Chapter 18
DP  - 2004
TI  - Local lymph node assays.
PG  - Unit18.2
LID - 10.1002/0471140856.tx1802s20 [doi]
AB  - The murine local lymph node assay (LLNA) allows identification of chemicals that 
      have the potential to cause skin sensitization and allergic contact dermatitis. 
      In this test, contact allergens are identified as a function of events occurring 
      during the induction phase of skin sensitization; specifically, stimulation of 
      proliferative responses in draining lymph nodes measured as a function of 
      radiolabeled thymidine incorporation in situ. Two methods are presented. In one, 
      lymph nodes are pooled on an experimental group basis, while in the other, lymph 
      nodes of individual animal are pooled, which enables statistical analyses. These 
      methods have been developed for contact sensitization hazard assessment and 
      provide a robust and reliable method as an alternative to guinea pig tests which 
      measure skin sensitizing potential as a function of challenge-induced skin 
      reactions in previously sensitized animals. It is also possible to use LLNA data 
      for measurement of relative potency in terms of risk assessment.
FAU - Dearman, Rebecca J
AU  - Dearman RJ
AD  - Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, 
      United Kingdom.
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Toxicol
JT  - Current protocols in toxicology
JID - 9816330
SB  - IM
MH  - Animals
MH  - Female
MH  - Lymph Nodes/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Toxicity Tests
EDAT- 2004/06/01 00:00
MHDA- 2013/02/26 06:00
CRDT- 2012/10/10 06:00
PHST- 2012/10/10 06:00 [entrez]
PHST- 2004/06/01 00:00 [pubmed]
PHST- 2013/02/26 06:00 [medline]
AID - 10.1002/0471140856.tx1802s20 [doi]
PST - ppublish
SO  - Curr Protoc Toxicol. 2004;Chapter 18:Unit18.2. doi: 10.1002/0471140856.tx1802s20.